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Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP8EFA
Plasmid#185854PurposeTesting a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationDHFR P66LPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
JL104: pMVP/Neo-DEST
Plasmid#121857PurposepMVP destination vector, empty expression vector backbone w/ Neo selection cassetteDepositorTypeEmpty backboneUsePmvp gateway destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH401: pMVP (L5-L4) CMV promoter
Plasmid#121726PurposepMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO102: pMVP (R4-R3) eGFP open
Plasmid#121731PurposepMVP R4-R3 entry plasmid, contains eGFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HJ601: pMVP (R4-R3) mCherry open
Plasmid#121733PurposepMVP R4-R3 entry plasmid, contains mCherry (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO601: pMVP (R4-R3) eCFP open
Plasmid#121735PurposepMVP R4-R3 entry plasmid, contains eCFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO401: pMVP (R4-R3) eYFP open
Plasmid#121737PurposepMVP R4-R3 entry plasmid, contains eYFP (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH305: pMVP (R4-R3) βgal open
Plasmid#121739PurposepMVP R4-R3 entry plasmid, contains βgal (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertβgal (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IY704: pMVP (L1-R5) EF1a promoter
Plasmid#121693PurposepMVP L1-R5 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LF801: pMVP (L1-R5) cTnT promoter
Plasmid#121696PurposepMVP L1-R5 entry plasmid, contains cTnT promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertcTnT promoter (Cardiomyocyte specific)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KU801: pMVP (L5-L4) mCherry
Plasmid#121709PurposepMVP L5-L4 entry plasmid, contains mCherry for 4-component MultiSite Gateway Pro assembly. Allows N-term mCherry fusion to gene of interest.DepositorInsertmCherry (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KU901: pMVP (L5-L4) eCFP
Plasmid#121711PurposepMVP L5-L4 entry plasmid, contains eCFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eCFP fusion to gene of interest.DepositorInserteCFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KV001: pMVP (L5-L4) eYFP
Plasmid#121713PurposepMVP L5-L4 entry plasmid, contains eYFP for 4-component MultiSite Gateway Pro assembly. Allows N-term eYFP fusion to gene of interest.DepositorInserteYFP (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5A1-T
Plasmid#92017PurposeExpresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator with 3 TEV cleavage sites
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…ExpressionMutationS219VPromoterAvailable sinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only