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Plasmid#228077PurposeMammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2h, S4h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3445 MTK3-mTagBFP2
Plasmid#226662PurposeMTK3 part containing the coding sequence of mTagBFP2DepositorInsertmTagBFP2
UseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Stargazin-EBFP2-FRB
Plasmid#222417PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertCacng2 (Cacng2 Mouse)
UseTagsEBFP2-HA-FRBExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-C-GRF4-P2A:mTagBFP2
Plasmid#216140PurposeControls for Bicistronic BiFCDepositorInsertGRF4 isoform 1
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-tagBFP
Plasmid#219965Purposeexpress TagBFP in mammalian cellsDepositorInsertTagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)
Plasmid#194217PurposeLentiviral transducition vector for a GAL4-responsive tagBFP reporter geneDepositorInsertUAS-ybTATA-tagBFP; PGK-mCitrine
UseLentiviralTagsExpressionMutationPromoterGAL4 UAS-ybTATA; PGKAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBI-BFP-NL1
Plasmid#206153PurposepBI-BFP-NL1DepositorInsertNeuroligin-1 (Nlgn1 Mouse)
UseTagsBicistronic BFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
SH4-FRB-EBFP2
Plasmid#222418PurposeTo express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertSH4
UseTagsFRB-HA-EBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBi2FC-C-MGGAGM-P2A:mTagBFP2
Plasmid#216141PurposeVector control for Bicistronic BiFCDepositorInsertMGGAGM Linker
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUBC-EBFP2
Plasmid#212314PurposeLentiviral backbone with EBFP2 reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-C1-SACM1LdeltaTMD-Fis1tail
Plasmid#220078PurposeSAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue FluorophoreDepositorInsertSACM1l-Fis1tail (SACM1L Human)
UseTagsExpressionMammalianMutationdeltaTMDPromoterAvailable sinceMay 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP-C1-FKBP-PIP5K1C-HD-EE
Plasmid#220090PurposeRecruitable PIP5K to make PIP2DepositorInsertPIP5K1C (PIP5K1C Human)
UseTagsTagBFP-FKBP1A(3-108)ExpressionMammalianMutationamino acids 1-635; D101R, R304D, R445E, K446EPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT310 AAV-BFPdonor
Plasmid#202057PurposeAAV HDR donorDepositorArticleInsertAAV-BFPdonor
UseAAV; Aav packaging plasmidTagsExpressionMammalianMutationITR deletionPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-insBFP.1
Plasmid#188025PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker insulin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; insulin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.1
Plasmid#188013PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-gcryBFP.2
Plasmid#188014PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker gamma crystallin BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; gamma crystallin promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.1
Plasmid#188019PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.2
Plasmid#188020PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GBH-2aG4-cmlc2BFP.3
Plasmid#188021PurposeCloning vector for conditional, intronic, targeted integration of a genebreak cassette optimized for zebrafishDepositorInsertTargeted 2aGal4VP16 protein trap; secondary marker cmlc2 BFP
UseTargetable insertional mutagen and reporter systemTagsExpressionMutationPromoterEndogenuous Promoter; cmlc2 promotorAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2-H3.1-WT-BFP
Plasmid#209437PurposeSB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFPDepositorInsertH3.1-WT
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only