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Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-sec:msfGFP
Plasmid#194914PurposeTetracycline inducible expression of msfGFP fused to Sec signal peptide in StaphylococciDepositorInsertShine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…TagsExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1
Plasmid#191484PurposeLentiviral expression of SARS-CoV-2 5' UTR ΔSL1DepositorInsertSARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SARS-CoV-2 5'UTR-Luciferase
Plasmid#191480PurposeLuciferase reporter for SARS-CoV-2 5' UTRDepositorInsertSARS-CoV-2 5' UTR
UseLentiviral and LuciferaseTagsExpressionMutationcontains SARS-CoV-2 5' UTRPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-BAP1-A95D-GFP
Plasmid#78914PurposeMammalian expression of BAP1 with A95D and an EGFP fusionDepositorInsertBAP1 (BAP1 Human)
UseTagsEGFP and HAExpressionMammalianMutationA95DPromoterCMVAvailable sinceJune 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
BCL9-BirA*
Plasmid#109147PurposeTo generate stable cell lines expressing BCL9-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertBCL9 (BCL9 Human)
UseTagsBirA(R118G)ExpressionMammalianMutationPromoterCMV/TetO2Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP2.GFP
Plasmid#109140PurposeGFP-tagged CYFIP2 expression constructDepositorInsertCYFIP2 (CYFIP2 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX304-YAP1 _SH3bm
Plasmid#59141PurposeYAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1DepositorInsertYAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationSH3 domain deleted (aa278-290)PromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa597-1407
Plasmid#19146DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 597-1407 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa1400-1648
Plasmid#19145DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 1400-1648 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV HA hRB delta CDK + S612
Plasmid#58914Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restoredDepositorInsertRb (RB1 Human)
UseTagsHAExpressionMammalianMutationdelta CDK* with S612 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HTRA3_PDZ_1
Plasmid#103914PurposeProtein expression and purification of HTRA3 PDZ domainDepositorInsertHTRA3 (HTRA3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA
Plasmid#170999PurposeHiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A]
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationMAVS(tmd)[R537A]PromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
B9L-BirA*
Plasmid#109146PurposeTo generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cellsDepositorInsertB9L (Bcl9l Mouse)
UseTagsBirA(R118G) and FlagExpressionMammalianMutationPromoterCMV/TetO2Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-Zeo-t2A-sHRPaNLG1
Plasmid#79914PurposepiggyBac transposon vector, Zeocin-2A-sHRPbNLG1DepositorInsertZeocin-t2A-splitHRP-aNLG1 (Nlgn1 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDcr-K70A.
Plasmid#89145PurposeExpression of helicase mutant of human Dicer in Sf9 cellsDepositorInserthDicer (DICER1 Human)
UseTagsHis6-tevExpressionInsectMutationK70APromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Dest-MBP-Nsp1
Plasmid#191483PurposeMammalian expression of MBP-tagged SARS-CoV-2 Nsp1DepositorInsertMBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only