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G273D HA-dynamin 1 pUHD10-3

Plasmid

#34689

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1 Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

G273D

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-HsTNRC6Biso1_1379-1723del1535-1624_P

Plasmid

#147204

Purpose

Bacterial Expression of HsTNRC6Biso1_1379-1723del1535-1624

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1379-1723del1535-1624 (TNRC6B Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation T1486A compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTOPO-rRNA-5'ETS 1 to 177

Plasmid

#23993

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA 5'ETS ntds 1 to 177

Use

Bacterial cloning - topo

Tags

Expression

Mutation

None

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
SpDJ-1 pET15b

Plasmid

#60689

Purpose

Recombinant protein expression of DJ-1

Depositor

Mark Wilson

Article

Madzelan et al FEBS J. 2012 Nov;279(22):4111-20. doi: 1

Insert

DJ-1 (SPAC22E12.03c Fission Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pLKO_FOXA1_#1

Plasmid

#70095

Purpose

Expression of shRNA to human FOXA1, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
wt HA-dynamin 1 pUHD10-3

Plasmid

#34688

Depositor

Sandra Schmid

Article

Damke et al J Cell Biol. 1995 Oct;131(1):69-80.

Insert

dynamin 1 (DNM1L Human)

Use

Tags

HA (variant)

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

Plasmid

#70659

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsTNRC6Biso1 1-1218_L

Plasmid

#146887

Purpose

Mammalian Expression of HsTNRC6Biso1_1-1218

Depositor

Elisa Izaurralde

Article

Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

Insert

HsTNRC6Biso1_1-1218 (TNRC6B Human)

Use

Tags

Expression

Mammalian

Mutation

4 non silent mutations, S441G, H1088R, Q1106R, Q1…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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His Puc (aa 1-393)

Plasmid

#52295

Purpose

Bacterial expression of His tagged Drosophila Puc fragment

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Insert

Puc (puc Fly)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 p37 SHPmut

Plasmid

#113503

Purpose

Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

F215A/E218A/QKL220-222AAA

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMT2 Alpha2 delta-1 V6

Plasmid

#89894

Purpose

Mammalian expression of Alpha2 delta-1 V6

Depositor

Annette Dolphin

Article

Kadurin et al Elife. 2016 Oct 26;5. pii: e21143. doi:

Insert

Cacna2d1 (Cacna2d1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Amino acids L943V, E944V, A945V, E947V, M948V are…

Promoter

adenovirus major late promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1 p37 deltaUBX

Plasmid

#113504

Purpose

Bacterial expression of GST-tagged p37 lacking the UBX domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Use

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 251-331 (stop codon behind AA250)

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS2/3

Plasmid

#91318

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS2/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN1-1/5-IS3/3

Plasmid

#91560

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS3/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ITSN1-1/5-IS1/3

Plasmid

#91429

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-1/5-IS1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-1/5-IS1/3 (ITSN1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-1

Plasmid

#136385

Purpose

Expresses DANCER-1 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-1

Use

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLacI-BCD7-Citrine P42U18C68T11 v40-1 (536)

Plasmid

#70725

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-BCD7-Citrine P62U18C68T11 v40-1 (523)

Plasmid

#70726

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBetI-BCD7-Citrine P69U18C68T11 v40-1 (532)

Plasmid

#70723

Purpose

Citrine reporter for transfer fxns

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge