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Gal1/10 His6 TEV Ade S. cerevisiae expression vector (12ADE-B)

Plasmid

#48298

Depositor

Scott Gradia

Type

Empty backbone

Use

Na

Tags

His6

Expression

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en31FnCas9ABEmax8.17d

Plasmid

#201956

Purpose

Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-SpCas9

Plasmid

#130968

Purpose

3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

3xHA-NLS-SpCas9

Use

CRISPR

Tags

3x HA, EGFP, and NLS

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en31FnCas9

Plasmid

#201949

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en31FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

G1243T, E1369R, E1603H on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en1FnCas9

Plasmid

#201947

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en1FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1369R on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-SpCas9-HF1

Plasmid

#201952

Purpose

Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9ABEmax8.17d

Plasmid

#201955

Purpose

Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-en15FnCas9

Plasmid

#201948

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en15FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1603H on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9

Plasmid

#130969

Purpose

3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

FnCas9

Use

CRISPR

Tags

3xHA-NLS and NLS-T2A-EGFP

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PBK-PRT100AH

Plasmid

#44739

Depositor

S. Anil Kumar, PB Kishor, P. Hima Kumari

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFE-Selo-FI-prk

Plasmid

#162698

Purpose

Expresses S. elongatus Form IB rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

S. elongatus Form IB rubisco and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63

Plasmid

#203331

Purpose

episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

Plasmid

#48313

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Sumo

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171385

Purpose

Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized minidCas13X.1-RESCUE-S

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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