Skip to main content
Addgene
Showing: 421 - 440 of 53929 results

  1. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid
    #47868
    Purpose
    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.
    Depositor
    James Thomson, Erik Sontheimer
    Article
    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.
    Inserts
    NmCas9
    U6pr-tracrRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Sept. 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    47868-plasmid-map-sequence-id-68833

    Enlarge

  5. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations (NT51)

    Plasmid
    #49061
    Purpose
    Expresses human NKCC1 mutated Ser and Thr residues in the N-terminus and an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    S150N, S155Q, S170Q, T177A, S183R, S193E, T203A, …
    Promoter
    CMV
    Available since
    Nov. 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    49061-plasmid-map-sequence-id-72183

    Enlarge

Showing: 421 - 440 of 53929 results