Addgene

We narrowed to 1,445 results for: MBP

Showing: 841 - 880 of 1445 results

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SARS-CoV-2_N_deltaCTD

Plasmid

#157871

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaCTD

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNTD

Plasmid

#157869

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N terminal domain

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaNTD

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaNIDR

Plasmid

#157868

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaNIDR

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM2

Plasmid

#120385

Purpose

Bacterial expression of MBP-Pumillio2 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM2 (PUM2 Human)

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCIDR

Plasmid

#157872

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Use

Tags

MBP

Expression

Bacterial

Mutation

deltaCIDR

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (WT)

Plasmid

#175419

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pmalc4x_I499F_TEV

Plasmid

#47703

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with I499F mutation (MYOC Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed I499F

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas7/pHMGWA

Plasmid

#81184

Purpose

Expresses Desulfovibrio vulgaris Cas7 in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas5c/pHMGWA

Plasmid

#81182

Purpose

Expresses Desulfovibrio vulgaris Cas5c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas5c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cas8c/pHMGWA

Plasmid

#81183

Purpose

Expresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Insert

Cas8c

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (H348E)

Plasmid

#175420

Purpose

Encodes His-MBP-TEV-ybbR-H348E eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Use

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N, H348E

Promoter

T7

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pmalc4x_G252R_TEV

Plasmid

#47704

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with G252R mutation (MYOC Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed Glycine 252 Arginine

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pmalc4x_K398R_TEV

Plasmid

#47701

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with K398R mutation (MYOC Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

changed Lysine 398 to Arginine

Promoter

Ptac

Available since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pMAL-7xHis-TEV-hexa

Plasmid

#222868

Purpose

Expresses His-tagged mutant (C19V/L56V/C110V/C130S/S135G/S219V) TEV protease in E. coli

Depositor

Nikolaus Loening

Article

Davis et al Protein Sci. 2025 Mar;34(3):e70049. doi:

Insert

TEV protease

Use

Tags

7xHis and MBP

Expression

Bacterial

Mutation

C19V, L56V, C110V, C130S, S135G, S219V. Insert in…

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM1

Plasmid

#120384

Purpose

Bacterial expression of MBP-Pumillio1 fusion protein

Depositor

Igor Ulitsky

Article

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM1 (PUM1 Human)

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

LacI

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-mF(3230)A3

Plasmid

#168016

Purpose

Expresses MBP-F(3230)A3-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

MBP-F(3230)A3-6xHis

Use

Tags

Hisx6 and maltose binding protein

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-mBoNT/F

Plasmid

#168015

Purpose

Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

MBP-BoNT/F-LC(1-428)-6xHis

Use

Tags

Hisx6 and maltose binding protein

Expression

Bacterial

Mutation

Promoter

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAW-AlphaLobe

Plasmid

#113255

Purpose

E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

Depositor

Jennifer Doudna

Article

Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

S. pyogenes Cas9 alpha-helical lobe

Use

Tags

10xHis-MBP

Expression

Bacterial

Mutation

residues 56–714; See paper for description

Promoter

T7

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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RC(WW)-LgBit

Plasmid

#208931

Purpose

Recognition domain for in vitro MAPK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

WW domain of PIN1 (PIN1 Human)

Use

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Use

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Article

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
HM3C NaVAe1_p pET24b

Plasmid

#127854

Purpose

Bacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1p

Use

Tags

3C Cleavage site, His, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD117A

Plasmid

#218141

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD40

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXPHM3-PCFT_NB

Plasmid

#165415

Purpose

Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT_NB (SLC46A1 Chicken, Llama)

Use

Tags

His10-MBP-3C protease site

Expression

Bacterial

Mutation

Promoter

arabinose

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HM3C NaVSp1_p pET24b

Plasmid

#127852

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Use

Tags

3C Cleavage site, His Tag, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPAB X2-FL

Plasmid

#216515

Purpose

Expresses MBP-tagged full length hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2 FL

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPAB X2-IDR

Plasmid

#216517

Purpose

Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-IDR

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-225

Promoter

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM90

Plasmid

#226682

Purpose

Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

Rpn5 (RPN5 Budding Yeast)
Rpn6 (RPN6 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)
Rpn9 (RPN9 Budding Yeast)
Rpn11 (RPN11 Budding Yeast)

Use

Tags

MBP-HRV

Expression

Bacterial

Mutation

S111TAG

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD103D

Plasmid

#218134

Purpose

For competition during selection with hybrid circuit. Expresses MBP fused to evolved degron SD55

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD55

Use

Tags

MBP

Expression

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
VF-Sensor

Plasmid

#208927

Purpose

in vitro RSK-Sensor for luminescent detection of RSK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

SOS1 (SOS1 Human)

Use

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PDE4B-Sensor

Plasmid

#208929

Purpose

in vitro JNK-Sensor for luminescent detection of JNK-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

PDE4B docking sequence+Phospho site and Smbit (PDE4B Human)

Use

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBXPHM3-NB-D6

Plasmid

#167989

Purpose

expression of a nanobody against rat PepT2 as a n terminal MBP fusion

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

NanobodyD6

Use

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2X-ORF24-NTD

Plasmid

#138465

Purpose

Expresses MBP-tagged ORF24-NTD in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

ORF24 (ORF24 KSHV (HHV-8))

Use

Tags

MBP

Expression

Bacterial

Mutation

Amino acids 1-201

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hnRNPAB X2-RBD

Plasmid

#216516

Purpose

Expresses MBP-tagged RNA binding domain of hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-RBD

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-64 and 226-304

Promoter

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSD118A

Plasmid

#218140

Purpose

Expresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coli

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

SD0_ZF2-3

Use

Tags

AviTag-6xHis-MBP-TEV-(GGGGS)2

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
RC(14-3-3ε)-LgBit

Plasmid

#208926

Purpose

Recognition domain for in vitro RSK-activity sensor

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

14-3-3ε (YWHAQ Human)

Use

Tags

HISx6, LgBit, and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HM3C NaVSp1 pET24b

Plasmid

#128088

Purpose

Bacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel protein

Depositor

Dan Minor

Article

Shaya et al Proc Natl Acad Sci U S A. 2011 Jul 26;10

Insert

NaVSp1p

Use

Tags

3C Cleavage, His Tag, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 841 - 880 of 1445 results

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