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Plasmid#191383PurposeDoxycycline inducible expression of RNA A-to-I editor rABEDepositorInsertrABE-Flag
UseLentiviral and Synthetic BiologyTagsFlagExpressionMammalianMutationPromotertet-inducible promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAVS-Mito
Plasmid#44556PurposeRetroviral vector for expression of a chimeric MAVS allele targeted to mitochondria in mammalian cellsDepositorInsertMAVS-Fis1 chimeric protein (MAVS Human)
UseRetroviralTagsExpressionMammalianMutationcontains aa1-500 of MAVS and aa127-152 of Fis1PromoterAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-mTurquoise2
Plasmid#98886PurposeProduces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRETDepositorInsertmNeonGreen-mTurquoise2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-dWPRE
Plasmid#60907PurposeThe plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imagingDepositorInsertscFv-GCN4
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry
Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIRE4-Azi
Plasmid#105829PurposePlasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cellsDepositorInsertsHumanized EAziRS
4 copies of the U6-BstYam tRNA cassette
UseTagsExpressionMammalianMutation(Y37L, D182S, F183M, L186A, D265R)TyrRSPromoterPGK and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
Plasmid#60910PurposedCas9 fused to 24 copies of the GCN4 peptide v4, which is part of the SunTag systemDepositorInsertdCas9
UseCRISPR and LentiviralTags2x NLS, HA, and NLSExpressionMammalianMutationPromoterdSV40 PromoterAvailable sinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmVen-RPA70-IRES-RPA32-IRES-RPA14
Plasmid#211431Purposemammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14DepositorUseTagsmVenus (on RPA70)ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-P2A-GFP
Plasmid#180020PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-EGFP markerDepositorInsertPEmax-P2A-EGFP
UseTagsP2A-EGFP, SV40 bpNLS, and c-Myc NLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only