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pWN_U6-TRAC-miniGag

Plasmid

#228960

Purpose

miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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LRCherry2.1

Plasmid

#108099

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA5-mRuby3

Plasmid

#184891

Purpose

Stable expression of ANXA5-mRuby3 (Annexin A5-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA5 (ANXA5 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pHBV-X/EnhI-Luc

Plasmid

#71418

Purpose

To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV X promoter and enhancer I driving Luciferase expression

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

HBV X

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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Level 1 P1 OsActinP:Hygint:NosT

Plasmid

#165423

Purpose

GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

Depositor

John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

Article

Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

Insert

Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator

Use

Synthetic Biology; L1 p1(r) golden gate (moclo) h…

Tags

Expression

Mutation

Promoter

Os Actin promoter :: Hygromycin resistance gene (…

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pWZL N-Myc-hPOT1_deltaOB

Plasmid

#166408

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

ΔOB

Promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA6-mRuby3

Plasmid

#184892

Purpose

Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA6 (ANXA6 Human)

Use

Tags

mRuby3

Expression

Mammalian

Mutation

R587K

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pQmod2C-GG

Plasmid

#191347

Purpose

Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDUAL_2_araBAD

Plasmid

#155176

Purpose

see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgGAL4

Plasmid

#121514

Purpose

sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgGAL4

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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TRE-KRAB-dCas9-IRES-GFP

Plasmid

#85556

Purpose

2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

Depositor

Eric Lander

Article

Fulco et al Science. 2016 Sep 29. pii: aag2445.

Insert

KRAB-dCas9-IRES-GFP

Use

CRISPR and Lentiviral

Tags

2x NLS, HA Tag, and KRAB domain

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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LBR-mCherry

Plasmid

#172446

Purpose

Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

lamin B receptor (LBR Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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iNos-eGFP

Plasmid

#207251

Purpose

Reporter for expression of eGFP under control of iNos promoter

Depositor

Michelle Farkas

Article

Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

Insert

iNos Promoter (Nos2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a - Ec-ClpX

Plasmid

#111516

Purpose

Expresses E. coli ClpX with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

ClpX

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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LRG2.1_Puro

Plasmid

#125594

Purpose

Lentiviral expression of sgRNA with GFP and puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pUWR-Cad-Venus

Plasmid

#58328

Purpose

Destination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

Cad-Venus

Use

Tags

Expression

Insect

Mutation

Promoter

poly-ubiquitin

Available since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only
p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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