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Plasmid#195734PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#20 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mini v2-HF24_F5
Plasmid#195719PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#5 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-E2302AI2313R_U
Plasmid#147734PurposeMammalian Expression of HsNot1iso2-E2302AI2313RDepositorInsertHsNot1iso2-E2302AI2313R (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-R2097DI2313R_U
Plasmid#147735PurposeMammalian Expression of HsNot1iso2-R2097DI2313RDepositorInsertHsNot1iso2-R2097DI2313R (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-del698-725-dsRNAres_U
Plasmid#147718PurposeInsect Expression of DmNot3-del698-725-dsRNAresDepositorInsertDmNot3-del698-725-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-del698-725-F764E-dsRNAres_U
Plasmid#147719PurposeInsect Expression of DmNot3-del698-725-F764E-dsRNAresDepositorInsertDmNot3-del698-725-F764E-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-del723-745-dsRNAres_U
Plasmid#147720PurposeInsect Expression of DmNot3-del723-745-dsRNAresDepositorInsertDmNot3-del723-745-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-3xmut_U
Plasmid#147731PurposeMammalian Expression of HsNot1iso2-3xmutDepositorInsertHsNot1iso2-3xmut (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso2-8xmut_U
Plasmid#147733PurposeMammalian Expression of HsNot1iso2-8xmutDepositorInsertHsNot1iso2-8xmut (CNOT1 Human)
UseTagsExpressionMammalianMutation4 silent and two non silent G917E and R2353Q muta…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-del698-725-F764E-dsRNAres_U
Plasmid#147704PurposeInsect Expression of DmNot3-del698-725-F764E-dsRNAresDepositorInsertDmNot3-del698-725-F764E-dsRNAres (Not3 Fly)
UseTagsExpressionInsectMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-6x mut-dsRNAres_U
Plasmid#147708PurposeInsect Expression of DmNot1-6xmut-dsRNAresDepositorInsertDmNot1-6xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-7xmut-dsRNAres_U
Plasmid#147709PurposeInsect Expression of DmNot1-7xmut-dsRNAresDepositorInsertDmNot1-7xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and three non silent N605S, K…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-8xmut-dsRNAres_U
Plasmid#147710PurposeInsect Expression of DmNot1-8xmut-dsRNAresDepositorInsertDmNot1-8xmut-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and three non silent N605S, K…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1967-2251-dsRNAres_U
Plasmid#147711PurposeInsect Expression of DmNot1-del1967-2251-dsRNAresDepositorInsertDmNot1-del1967-2251-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso2-del1844-2133_T
Plasmid#147622PurposeMammalian Expression of HsNot1iso2-del1844-2133DepositorInsertHsNot1iso2-del1844-2133 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot3-del607-634-F673E_S
Plasmid#147503PurposeMammalian Expression of HsNot3-del607-634-F673EDepositorInsertHsNot3-del607-634-F673E (CNOT3 Human)
UseTagsExpressionMammalianMutationtwo silent mutations compared to the sequence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-del1844-2133-V1251R_S
Plasmid#147484PurposeMammalian Expression of HsNot1iso1-del1844-2133-V1251RDepositorInsertHsNot1iso1-del1844-2133-V1251R (CNOT1 Human)
UseTagsExpressionMammalianMutationDeletion of 5 AA compared to the sequence given b…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-mini v2-HF24_F12
Plasmid#195726PurposeThis plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.DepositorInsertFragment#12 of 24 fragment splits in lacI/lacZ cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC
Plasmid#148404PurposeInsect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNaresDepositorInsertDmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1318-1605-shRNAres_AC
Plasmid#148414PurposeMammalian Expression of HsNot1iso1_1318-1605-shRNAresDepositorInsertHsNot1iso1_1318-1605-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only