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Plasmid#82879PurposeGateway Donor vector containing CGREF1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertCGREF1 (CGREF1 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_AKT3_p.E17K
Plasmid#82172PurposeGateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.DepositorInsertAKT3 (AKT3 Human)
UseGateway entry vectorTagsExpressionMutationE17KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_FTH1_p.A161T
Plasmid#81278PurposeGateway Donor vector containing FTH1 , part of the Target Accelerator Plasmid Collection.DepositorInsertFTH1 (FTH1 Human)
UseGateway entry vectorTagsExpressionMutationK88E, L98Q, A161TPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag DAK
Plasmid#20473DepositorInsertDAK (DAK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196084Purpose(Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196078Purpose(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX305_FKBP12F36V-SHOC2
Plasmid#134522PurposeLentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsFKBP12F36V-2xHAExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterhPGKAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196076Purpose(Empty Backbone) Constitutive CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196082Purpose(Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Aminoglycoside phosphotransferase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag FGFR1
Plasmid#20486DepositorInsertFGFR1 (FGFR1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
SHP2(WT)@pLKO-Trex-SBP
Plasmid#85457PurposeGene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
SHP2(C459S)@pLKO-Trex-HA
Plasmid#85459PurposeExpresses SHP2 mutant in mammalian cellsDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsHAExpressionMammalianMutationC459SPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196080Purpose(Empty Backbone) Inducible CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
Blasticidin S deaminase
UseCRISPRTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag GCK
Plasmid#20492DepositorInsertGCK (GCK Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196074Purpose(Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
UseTagsExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AKT3
Plasmid#20423DepositorInsertAKT3 (AKT3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HSD17B10_WT_V5
Plasmid#82941PurposeGateway Donor vector containing HSD17B10, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorInsertHSD17B10 (HSD17B10 Human)
UseGateway entry vectorTagsExpressionMutationPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
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SHP2(T253M/Q257L)@pLKO-Trex-SBP
Plasmid#85458PurposeSHP2 mutant gene expressionDepositorInsertSHP2 (PTPN11 Human)
UseLentiviralTagsSBP TagExpressionMammalianMutationT253M/Q257LPromoterRSVAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only