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pLX307 LacZ

Plasmid

#117730

Purpose

Open reading frame vector encoding LacZ

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

LacZ

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-puro LEF1

Plasmid

#27023

Depositor

Joan Massague

Article

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

LEF1 (LEF1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pCW57-GFP-miR-302cluster

Plasmid

#132549

Purpose

Doxycycline-inducible expression of human miR-302 cluster

Depositor

Tomáš Bárta

Article

Peskova et al Sci Rep. 2019 Oct 31;9(1):15759. doi: 10

Insert

human miR-302 cluster: MIR302B, MIR302C, MIR302A, MIR302D, MIR367

Use

Lentiviral; Doxycycline inducible, microrna expre…

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP spGFP-ERGIC53

Plasmid

#38270

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

ERGIC-53 (LMAN1 Human)

Use

Retroviral

Tags

EGFP with Calreticulin signal peptide at N-termin…

Expression

Mammalian

Mutation

deleted signal peptide (1-30 amino acids)

Promoter

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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p7141 pHAGE-P-CMVt-N-HA-GAW-MUS81

Plasmid

#100157

Purpose

Expresses MUS81

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

MUS81 (MUS81 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIP-mCherry-CDMPR

Plasmid

#182643

Purpose

Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), followed by mCherry and murine CDMPR (without its signal peptide)

Depositor

Martin Spiess

Article

Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

Insert

CDMPR

Use

Retroviral

Tags

N-terminal ER targeting sequence of hemagglutinin…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-MLPmin-luc2

Plasmid

#194383

Purpose

Reporter plasmid for split TEV assays; firefly luciferase reporter gene driven by 10x clustered UAS binding sites linked to a minimal MLP promoter

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

10xUAS-luc2

Use

Tags

Expression

Mammalian

Mutation

Promoter

MLP minimal

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-EGFP-GPI

Plasmid

#65298

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-HA-mouse GARP

Plasmid

#180258

Purpose

Mouse full length GARP expression

Depositor

Timothy Springer

Article

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

GARP (Lrrc32 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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attP-puro-GA donor

Plasmid

#182140

Purpose

attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Luciferase

Plasmid

#99283

Purpose

All-in-one lentivector with tet-inducible luciferase

Depositor

Stephen Tapscott

Article

Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

Insert

Firefly luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Reporter-2

Plasmid

#180219

Purpose

Surrogate reporter plasmid for the assay of targeted RNA editing

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Insert

Surrogate reporter

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPGKpuro

Plasmid

#11349

Depositor

Rudolf Jaenisch

Article

Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

Insert

pgk-1 5' and 3' regions (Pgk1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJMK074: CMV QuasAr2-TS-GCaMP6f

Plasmid

#72303

Purpose

Combines a gCaMP-based calcium indicator and an Arch-based voltage indicator

Depositor

Adam Cohen

Article

Dempsey et al J Pharmacol Toxicol Methods. 2016 May 13

Insert

CaViar

Use

Lentiviral

Tags

TS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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pAS_8xMmaPylT_EF1_FLAG-MmaPylRS

Plasmid

#174890

Purpose

Mma PylRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

van Husen et al Chembiochem. 2021 Aug 25. doi: 10.1002/c

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

U25C PylT

Promoter

EF1

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-NLuc_SV40:PuroR

Plasmid

#183045

Purpose

Expresses FLAG-tagged NanoLuciferase (NLuc-only) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

NanoLuciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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AsdCas12a VPR

Plasmid

#188512

Purpose

Expresses FLAG tagged AsdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MORC2 ts1

Plasmid

#115886

Purpose

MORC2 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MORC2 shRNA (MORC2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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FEN1 C10.3 gRNA

Plasmid

#90689

Purpose

3rd generation lentiviral gRNA plasmid targeting human FEN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FEN1 (Guide Designation C10.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLV.ATMi

Plasmid

#14542

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATM shRNA (ATM Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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