-
Plasmid#158393PurposeGolden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
-
pET-28c(+)-YTHDF3
Plasmid#102276PurposeFor bacterial expression of His-tagged human YTHDF3DepositorInsertYTHDF3 (YTHDF3 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 p53 WT
Plasmid#69003Purposemammalian expression of wild type human p53DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHrD-IRES-Fluc
Plasmid#194250PurposeHuman RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutationPromoterhuman rDNA promoter and beginning of 5' ETS ā¦Available sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergilluā¦Available sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132-tRNA2.0
Plasmid#158394PurposeGolden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.sgRNA.EFS.tRFP
Plasmid#57823Purpose3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter drivenDepositorInsertssgRNA
EFS
tagRFP
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDIV301
Plasmid#204969PurposeAMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance markerDepositorInsertsMad7
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergilluā¦Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV300
Plasmid#204968PurposeAMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance markerDepositorInsertsMad7
hph (hygromycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAspergillus nidulans tef1 promoter and Aspergilluā¦Available sinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-BFP
Plasmid#85449PurposeLentiviral vector expressing a KRAB-dCas9 fusion protein and BFPDepositorInsertKRAB-dCas9-IRES-BFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianMutationPromoterTRE3GAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-His3MX6-PGAL1-GFP
Plasmid#41616DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a geneā¦ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW63.YY1.FKBP.knock-in.BFP
Plasmid#104371PurposeFor knocking in FKBP F36V P2A BFPDepositorInsertFKBP12(F36V) -P2A-BFP
UseTagsExpressionMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136-tRNA2.0
Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-eGFP-OPTN
Plasmid#175750PurposeLentiviral vector expressing N-terminally tagged eGFP-OPTNDepositorInsertOPTN (OPTN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf R401H
Plasmid#118334PurposeExpression of human CrafDepositorInsertCraf R401H (RAF1 Human)
UseTagsFlagExpressionMammalianMutationR401HPromoterCMVAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf SSDD
Plasmid#118337PurposeExpression of human CrafDepositorInsertCraf SSDD (RAF1 Human)
UseTagsFlagExpressionMammalianMutationSSDDPromoterCMVAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only