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Plasmid#120574PurposeExpresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorInsertNRAS (NRAS Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag AAK1
Plasmid#20133DepositorInsertAAK1 (AAK1 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
p2xFlag CMV2 Lats1-PY1+2 mutant
Plasmid#18975DepositorInsertLarge tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
UseTags2xFlagExpressionMammalianMutationPY1 mutant, (Tyrosine 376 changed to Alanine) and…PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Tight-FLAG-CYLD-C601A
Plasmid#60029PurposeCYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tagDepositorInsertCylindromatosis (CYLD Human)
UseTagsFLAGExpressionMammalianMutationCysteine 601 changed to AlaninePromoterTREAvailable sinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag ADRBK1
Plasmid#20418DepositorInsertADRBK1 (GRK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMT[Hygro]_GBP-3xFlag-DEST
Plasmid#157813PurposeA gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selectionDepositorInsertGFP-binding protein
UseFor drosophila cellsTags3xFlagExpressionInsectMutationPromotermetallothioneinAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S199D][S227D]-FLAG
Plasmid#199324Purposedouble mutated phosphorylation site (kinase acitivity and MLKL interaction active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 199 and 227 to Aspartic AcidPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-puro-Venus-FLAG-CD59
Plasmid#50377PurposeExpress Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertVenus
UseTagsExpressionMammalianMutationPromoterSR alphaAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73
Plasmid#111724PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ73 (polyQ repeat)PromoterCMV and P10Available sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (F118D) FLAG GFP
Plasmid#164979PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationF118DPromoterUBCAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag NEK6
Plasmid#20548DepositorInsertNEK6 (NEK6 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
1354 pcDNA3 flag FKHR deltaDB
Plasmid#10694DepositorInsertFKHR deltaDB (FOXO1 Human)
UseTagsflagExpressionMammalianMutationdeletion of amino acids 208-220PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG
Plasmid#136513PurposeExpresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cellsDepositorInsertcodon-optimized AcrIIA5
UseTagsFLAG/NLSExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PCK2
Plasmid#20559DepositorInsertPCK2 (PCK2 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag NEK3
Plasmid#20547DepositorInsertNEK3 (NEK3 Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXJ40-Flag-Ferritin-iBox-PAK4cat
Plasmid#190180Purposeexpresses Flag-tagged Ferritin-iBox-PAK4catDepositorInsertFerritin-iBox-PAK4cat
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -