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Plasmid#104531Purposebacterial expression of lambda exonucleaseDepositorInsertλ exonuclease
UseTagsHis6 followed by a thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s
Plasmid#105715PurposeIn the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.DepositorHas ServiceAAV5Insertsynaptophysin-GCaMP6s
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1a, Human elongation factor-1 alphaAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-Rncp-iGluSnFR1
Plasmid#107336PurposeRed fluorescent glutamate sensor with ncp topology. Membrane-displayedDepositorInsertRncp-iGluSnFR1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRATIO3212
Plasmid#141311PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
ROPY (sYFP2-OP18-mScI-R125I)
Plasmid#128002PurposeImproved redshifted FRET-readout of Stathmin/OP18 interaction with α-tubulinDepositorInsertsYFP2-OP18-mScI-R125I
UseTagsExpressionMammalianMutationR125I in mScarlet-IPromoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4214
Plasmid#141314PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmCerulean and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-EGFP
Plasmid#140877Purpose126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.DepositorInsertEGFP
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-EGFP-ssHRP-TM
Plasmid#31156DepositorInsertssHRP-TM
UseMembrane targeted hrp (mhrp)TagsExpressionMammalianMutationincludes signal sequence and a transmembrane doma…PromoterCMV promoter including immediate early promoter/e…Available sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-T
Plasmid#51232Purposebi-directional expression vector for yeast, constitutively active, TRP1 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-VP16 mouse MyoD-T2A-dsRedExpress2
Plasmid#60626PurposeExpresses VP16 mouse MyoD and dsRedExpress2 in response to doxycyclineDepositorInsertVP16 mouse MyoD
UseLentiviralTagsExpressionMutationPromoterTREAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-U
Plasmid#51230Purposebi-directional expression vector for yeast, constitutively active, URA3 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L
Plasmid#52481PurposeExpresses mCherry, EGFP and Synaptophysin-TagRFP-TDepositorInsertsmCherry
EGFP
Synaptophysin-TagRFP-T
UseRabies virusTagsTagRFP-TExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS-PluxIlux
Plasmid#47641Purposewt luxI promoter in pCS26DepositorInsertPluxI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPluxIAvailable sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hNox5
Plasmid#69354Purposeexpresses human Nox5 in mammalian cellsDepositorInserthNox5 (NOX5 Human)
UseTagsnoExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2214
Plasmid#141303PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmCerulean and mScarlet-IExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMP107
Plasmid#207925PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-L
Plasmid#51226Purposebi-directional expression vector for yeast, constitutively active, LEU2 selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterGPD/ADH1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-9xSeq2-Luc
Plasmid#42214PurposeLuciferase reporter for GI-ZFP2DepositorInsert9xSeq2 Binding Sites
UseLuciferaseTagsExpressionMammalianMutationPromoter9 tandem copies of Seq2 + minimal human CMV promo…Available sinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMP108
Plasmid#207928PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M:FastRed, for braidingDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV566 A-USF
Plasmid#33360DepositorInsertUSF (Usf1 Mouse)
UseTagsHAExpressionMammalianMutationDominant Negative (see notes below)PromoterCMVAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only