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MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Use

Tags

Expression

Mutation

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-HxB.S

Plasmid

#65415

Purpose

contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

Depositor

Harold Erickson

Article

Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

Insert

tenascin C small splice variant (TNC Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G178E

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCoofy50

Plasmid

#55189

Purpose

Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

8x His, FLAG, HA, PreScission site (3C protease s…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pCMrfp

Plasmid

#59773

Purpose

Derived from pCM62; ampR, tetR, PBAD - rfp

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

REV1

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-Lifeact-GFP

Plasmid

#175437

Purpose

F-actin structures labelling

Depositor

Sebastián Dupraz, Frank Bradke

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lifeact-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

pTub-alpha1

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTSTEP

Plasmid

#72334

Purpose

Tissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)

Depositor

Avital Rodal

Article

Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.

Insert

RRS-TagRFPT-p10-[Golic+] RRS-EGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2+ Bmi1-cherry-HA

Plasmid

#30516

Depositor

John Gurdon

Article

Pasque et al EMBO J. 2011 May 6. ():.

Insert

Bmi1 (Bmi1 Mouse)

Use

Xenopus/zebrafish expression

Tags

cherry and triple HA

Expression

Mammalian

Mutation

NO STOP

Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Mutation

Promoter

polH (polyhedrin)

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Article

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pGMEH-P38-luxAB-ssrAec

Plasmid

#27062

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-ssrAec

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2011

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Neg. Ctrl_sgRNA

Plasmid

#111298

Purpose

CRISPR KO murine neg. ctrl.

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

NegCtrl sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+5'MT-wbp2nl

Plasmid

#172467

Purpose

expresses myc-tagged version of wild-type protein

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl

Use

Xenopus expression

Tags

Myc

Expression

Mutation

Promoter

SP6

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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