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pMpGWB210

Plasmid

#68601

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

3xFLAG

Expression

Plant

Mutation

Promoter

MpEF1alpha promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj12

Plasmid

#69942

Purpose

pSTC2,TheoHHAzRaj12,cisRaj12-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj12,cisRaj12,sfGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSCKtheoRaj11

Plasmid

#69939

Purpose

pSTC1,TheoHHAzRaj11,cisRaj11-sfGFP, kanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

TheoHHAzRaj11,cisRaj11,sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pSCKbreak12

Plasmid

#69938

Purpose

pSTC2,cisRaj12-sfGFP, KanR

Depositor

Alfonso Jaramillo

Article

Shen et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

CisRaj12-sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pGMCKq20-T38S38-0X

Plasmid

#49522

Purpose

Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

Depositor

Dirk Schnappinger

Article

Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

Insert

constitutive TetR38

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptb38

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1346 U6-B2M sgRNA Gag-pol v2

Plasmid

#201917

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Inserts

Gag-pol
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG and Human U6

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Article

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA3mt

Plasmid

#58219

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA3mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV CEPIA4mt

Plasmid

#58220

Purpose

Green fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

CEPIA4mt

Use

Tags

myc

Expression

Mammalian

Mutation

GCaMP2 N263Y E282V E334D M339L F395W D436E

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-GEM-GECO1

Plasmid

#32442

Purpose

Mammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 8.

Insert

GEM-GECO1

Use

Tags

Expression

Mammalian

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-p53DD

Plasmid

#22729

Depositor

Shinya Yamanaka

Article

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

Trp53 (Trp53 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

deleted amino acids 14-301

Promoter

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-EF1a-i53

Plasmid

#216243

Purpose

Express i53 in mammalian cells as a HDR enhancer

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

i53

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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