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Plasmid#174077PurposePlasmid with p15a origin of replication expressing codon optimized superfolder GFP-134/150TAG with C-terminal His6 tag, under T7 promoterDepositorInsertSuperfolder GFP
UseTagsHis-6ExpressionBacterialMutationD134TAG + N150TAGPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMN2E7-Fluc-Nluc-reporter
Plasmid#221005PurposeTransiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.DepositorInsertFluc-SMN2E7-STOP-Nluc
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry-MHC-IIA
Plasmid#35687DepositorInsertMHC IIA (MYH9 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
Piggybac-TetO-hNurr1-blast
Plasmid#176485PurposeTetO-hNurr1 in a PiggyBac vector with Blasticidin resistanceDepositorInsertNurr1 (NR4A2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-H2BGFP
Plasmid#26790DepositorInsertH2B-GFP (H2BC21 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
TurboRFP-pBAD
Plasmid#54858PurposeLocalization: Protein Expression Vector , Excitation: 553, Emission: 574DepositorTypeEmpty backboneUseTags6xHis and TurboRFPExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-anti-AlfaTag nanobody
Plasmid#171819PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInserttdTomato-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDULE-ABK
Plasmid#49086PurposeA pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experimentsDepositorInsertstRNA synthetase
tRNA for a photocrosslinking amino acid
UseTagsExpressionBacterialMutationL274M, C313A, Y349FPromoterGlnRS and lppAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-mWnt1
Plasmid#176486Purposemouse Wnt1 cDNA in TetO backboneDepositorInsertmouse Wnt1 cDNA (Wnt1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mem
Plasmid#55779PurposeThis red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.DepositorInsertmCherry-Mem
UseTagsThe N-terminal 20 residues of neuromodulin are fu…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pW211-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170809PurposeLentiviral vector to co-express an spsgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ841
Plasmid#39318DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterT7Available sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
UseTags6xHis and SEP tag (6xD)ExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10
Plasmid#126948PurposeBacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
HSA3_His
Plasmid#202765Purposehuman serum albumin design HSA3, in pET29b with C-terminal 6His-tagDepositorInsertHSA3
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFS478
Plasmid#89066PurposeKanMX Z3EV promoter cassetteDepositorInsertZ3EV promoter cassette
UseYeast integration vectorTagsExpressionMutationyeast Gal1 promoter modified with multiple Zif268…PromoterZ3EV promoterAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTcI39-switch
Plasmid#195557PurposeTemperature state switch expressing low-temperature mWasabi and high-temperature mRFP1DepositorInsertsmRFP1
mWasabi
TcI39
UseTags6x His and AANDENYADAS degradation tagExpressionBacterialMutationA67TPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRCC-N
Plasmid#81192PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETcLIC-GFP
Plasmid#183514PurposeThe plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coliDepositorTypeEmpty backboneUseTags10x His-tag and folding reporter GFPExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET21d-myc-BirA
Plasmid#109424PurposeBacterial expression of biotin ligase (BirA) with an N-terminal myc epitopeDepositorInsertBirA (birA )
UseTagsmycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RaACR_687-EYFP
Plasmid#111077PurposeAnion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cellsDepositorInsertAnion channelrhodopsin RaACR_687
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbGFP
Plasmid#166858PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a GFP-specific nanobody via a flexible linker. Derived from https://www.addgene.org/35363/.DepositorInsertcsgBACEFG, with GFP-specific nanobody fused to CsgA via flexible linker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA7002-avrPto
Plasmid#49156PurposePlant transformation binary vector for Dexamethasone inducible expression of the type III effector gene avrPtoDepositorInsertavrPto
UsePlant transformation binary vectorTagsExpressionMutationPromoterdexamethasone inducibleAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 C506S
Plasmid#78584PurposeExpression flag-USP19 C506S in mammalian cellsDepositorInsertflag-USP19 C506S (USP19 Human)
UseTagsflagExpressionMammalianMutationmutate 506 cysteine residue to serinePromoterCMVAvailable sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
BSA1_His
Plasmid#202766Purposebovine serum albumin design BSA1, in pET29b with C-terminal 6His-tagDepositorInsertBSA1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
HSA1_His
Plasmid#202763Purposehuman serum albumin design HSA1, in pET29b with C-terminal 6His-tagDepositorInsertHSA1
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-SpyCatcher
Plasmid#79053PurposeExpresses maltose binding protein (MBP) SpyCatcher fusionDepositorInsertMBP-SpyCatcher
UseTags6xHis and Maltose binding proteinExpressionBacterialMutationDerived from pDEST14-SpyCatcher (Plasmid #35044)…PromoterT7Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
KA0637_pPBCAG-rtTAM2-IN
Plasmid#124166PurposepiggyBac-based expression of rtTA-M2DepositorInsertrtTAM2-IRES-Neo
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-FEN1hWT
Plasmid#35027DepositorInsertFEN1 (FEN1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
USP19 sgRNA2
Plasmid#78586Purposedelete USP19 gene in human cellDepositorInsertUSP19 sgRNA2 (USP19 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1-CAG-Flex-FlpO-WPRE
Plasmid#171690PurposeCre-dependent expression of Flp-recombinaseDepositorInsertFlip-recombinase
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HA-UBA6
Plasmid#136995PurposeExpresses human UBA6 in mammalian cellsDepositorInsertUBA6 (UBA6 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
mNG2(1-10)_tdTomato
Plasmid#184169PurposeLentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.DepositorInsertsmNG2(1-10)
tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
GZnP2 in pcDNA3.1+
Plasmid#161739PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)DepositorInsertGZnP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PB1-AG(Y63L)-LOV2
Plasmid#178876PurposeMammalian expression of PB1-AzamiGreen(Y63L)-LOV2DepositorInsertPB1-AzamiGreen(Y63L)-LOV2
UseTagsExpressionMammalianMutationAzamiGreen: Y63LPromoterCAGAvailable sinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-iresFUCCI
Plasmid#133371PurposePiggybac vector for constitutive expressionDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL0426 (CRISPR with spc3)
Plasmid#130642PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.DepositorInsertCRISPR(spacer-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGene-TAP-GBP1
Plasmid#138087PurposePlasmid with TAP-GBP1 ORFDepositorInserthuman GBP1 (GBP1 Human)
UseTagsTandem affinity purification (TAP) tagExpressionMammalianMutationPromoterGal4-E1bAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTopo_Ex2Donor_pPGK-PURO
Plasmid#198862PurposeDonor template for CRISPR/Cas9 targeting of TERT Ex2DepositorInsertPURO
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO FAAA)3
Plasmid#130703PurposeMammalian expression plasmid for negative control of mCherry-(SUMO)3.DepositorInsert3 copies of human SUMO1 separated by (GGS)4
UseTagsExpressionMammalianMutationFKVK sequence in each SUMO1 module changed to FAAAPromoterCMVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only