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Showing: 4301 - 4320 of 5035 results

  9. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  15. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113035-plasmid-map-sequence-id-224728

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  16. InsRA-TagBFP

    Plasmid
    #79791
    Purpose
    Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.
    Depositor
    James Johnson
    Article
    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:
    Insert
    InsRA (INSR Human)
    Use
    Tags
    TagBFP extracellular
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 5, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    79791-plasmid-map-sequence-id-149402

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  19. CoV2-N-B.1.1.7

    Plasmid
    #177958
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D3L,R203K,G204R,S235F
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177958-plasmid-map-sequence-id-376958

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  20. CoV2-N-P.1

    Plasmid
    #177959
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    P80R,R203K,G204R
    Promoter
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177959-plasmid-map-sequence-id-348182

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Showing: 4301 - 4320 of 5035 results