Addgene

We narrowed to 13,763 results for: pET

Showing: 4301 - 4320 of 13763 results

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK17_amiE

Plasmid

#59837

Purpose

Constitutively expresses AmiE with proK17 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

proK17

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCri-8a-Strep

Plasmid

#61340

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pJK_pro6_eGFP

Plasmid

#59851

Purpose

Constitutively expresses eGFP with pro6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

pro6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK14_eGFP

Plasmid

#59848

Purpose

Constitutively expresses eGFP with proK14 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK14

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pAM248

Plasmid

#226892

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)_W166A/Y167A

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Use

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

W166A/Y167A

Promoter

T7

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM257

Plasmid

#226901

Purpose

6xHis-PSP-MBP-Pex3(40-441)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex3 (PEX3 Budding Yeast)

Use

Tags

6xHis-PSP-MBP

Expression

Bacterial

Mutation

amino acids 40-441

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM262

Plasmid

#226906

Purpose

20aa^Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Use

Tags

-PSP-6xHis-FLAG

Expression

Bacterial

Mutation

1-253

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM252

Plasmid

#226896

Purpose

MBP-Pex15(1-327)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Use

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-327))

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM245

Plasmid

#226889

Purpose

6xHis-PSP-GS-PANN(75-150)-(5aaGS)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Use

Tags

His-Precission-PANN

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM250

Plasmid

#226894

Purpose

Pex15(1-253)-PSP-6xHis-FLAG

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Use

Tags

prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-253)

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM251

Plasmid

#226895

Purpose

MBP-Pex15(1-309)-PSP-GSS-FLAG-6xHis

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

pex15 (PEX15 Budding Yeast)

Use

Tags

MBP and prescission -GSS-FLAG-6xHis

Expression

Bacterial

Mutation

Pex15(aa 1-309)

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM247

Plasmid

#226891

Purpose

6xHis-PSP-GS-Hcp1(1-162)-(6aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Use

Tags

His-Precission-Hcp

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM246

Plasmid

#226890

Purpose

6xHis-PSP-GS-ccHex-(9aaSG)-Msp1(36-362)

Depositor

Andreas Martin

Article

Castanzo et al Proc Natl Acad Sci U S A. 2020 Jun 30;11

Insert

msp1 (MSP1 Budding Yeast)

Use

Tags

His-Precission-cchex

Expression

Bacterial

Mutation

aa 36-362

Promoter

T7

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pQA-LP574

Plasmid

#222953

Purpose

Expresses R120A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 120 to Alanine

Promoter

T7

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM284

Plasmid

#226122

Purpose

Expression of cdc48 E588Q

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

E588Q

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM290

Plasmid

#226127

Purpose

Expression of His6-GST-3C-Npl4

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM289

Plasmid

#226126

Purpose

Expression of Npl4 D460K/Y461A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 D460K/Y461A (NPL4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

D460K/Y461A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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paM291

Plasmid

#226128

Purpose

Expression of His6-GST-3C-Npl4 T255A/T571A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 T255A/T571A (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

T255A/T571A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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