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Plasmid#17327DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E3D1_D73C
Plasmid#39328DepositorInsertMSP1E3D1_D73C (APOA1 synthetic gene)
UseTags6xHisExpressionBacterialMutationcys mutant of MSP1E3D1PromoterT7Available sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPdX
Plasmid#85084PurposeExpresses putidaredoxin in E. coliDepositorInsertPutidaredoxin
UseTagsHis6 tagExpressionBacterialMutationchanged Cysteine 73 to Serine and Cysteine 85 to …PromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
UseTags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ALIX-His(1-868, P801G)
Plasmid#186808PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).DepositorInsertALIX-His (1-868, P801G) (PDCD6IP Human)
UseTagsHis6ExpressionBacterialMutationProline 801 to GlycinePromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Danio rerio cryaba
Plasmid#47620PurposeRecombinant protein productionDepositorInsertAlpha Ba-crystallin (cryaba Zebrafish)
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12a
Plasmid#164658PurposeBacterial expression of LbCas12a using CL7/Im7DepositorInsertLbCas12a
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMH-macroHC
Plasmid#82337PurposeProvides a C-terminal macro domain and 6xHis tag for expression in E. coliDepositorInsertSPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
UseTags6xHis non-cleavable and macro domain of human his…ExpressionBacterialMutationTruncated protein, amino acids 1-178PromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK_kanR_proJK1_PAD
Plasmid#60630PurposeConstitutively expresses PAD (phenolic acid decarboxylase)DepositorInsertPhenolic acid decarboxylase padC (padC )
UseTagsExpressionBacterialMutationK146YPromoterproJK1Available sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSA-38
Plasmid#17328DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-GCN
Plasmid#127475Purposecoding sequence for synthetic zinc finger ZF-ADD-GCNDepositorInsertZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pST96/LBT-MCS
Plasmid#108227PurposeBacterial expression expression of protein with N-terminal lanthanide binding tagDepositorInsertLBT
UseTagsExpressionBacterialMutationPromoterT7, lacOAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pSRK51
Plasmid#223722PurposeExpresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coliDepositorInsertTEV protease
UseTagsNT* (self cleaving)ExpressionBacterialMutationL56V/S135G/S219VPromoterT7Available sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP580
Plasmid#222959PurposeExpresses R141S K142S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 141 to Serine and Lysine 142 to …PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQA-LP579
Plasmid#222958PurposeExpresses R36S K51S LoaP double mutantDepositorInsertantiterminator protein LoaP
UseTags10xHIS-SUMOExpressionBacterialMutationmutated Arginine 36 to Serine and Lysine 51 to Se…PromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
OmpT
Plasmid#213137PurposeOmpT_native membrane expressionDepositorInsertOmpT
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only