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pNMTMA_MBP_Z_Cav1_H6_Periplasmic_mScarlet

Plasmid

#223703

Purpose

To produce caveospheres with periplasmically encapsulated mScarlet

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP_Z_Cav1_H6_Periplasmic_mScarlet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRHA40

Plasmid

#138941

Purpose

XOR gate with IPTG and aTc as inputs and mRFP1 as output

Depositor

Guillermo Rodrigo

Article

Rosado et al PLoS Genet. 2018 Jul 19;14(7):e1007548.

Insert

riboregulated mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ1228

Plasmid

#11410

Depositor

Sung-Hou Kim

Article

Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

Insert

eukaryotic translation initiation factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
p663-UBC-PM-TurboID-V5_IDG-K

Plasmid

#214455

Purpose

Gateway destination clone of PM-TurboID (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PM-TurboID-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLPT107

Plasmid

#85525

Purpose

Repressilator with triple reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI
mKate2

Use

Synthetic Biology

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLPT119

Plasmid

#85526

Purpose

Repressilator without degradation tags, with integrated reporter

Depositor

Johan Paulsson

Article

Potvin-Trottier et al Nature. 2016 Oct 12;538(7626):514-517. d

Inserts

mCfp
mVenus
tetR
lacI
cI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFIP

Plasmid

#17565

Depositor

Jay Keasling

Article

Ham et al Biotechnol Bioeng. 2006 May 5. 94(1):1-4

Insert

inversion switch

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pAID5.1-N

Plasmid

#145808

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPkan-S2

Plasmid

#61601

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Tg(lyz:EGFP-Mmu.Rpl10a)

Plasmid

#128838

Purpose

RNA isolation from neutrophils via TRAP

Depositor

Anna Huttenlocher

Article

Lam et al PLoS One. 2013 Dec 19;8(12):e84436. doi:

Insert

EGFP-L10a

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAID5.1-C

Plasmid

#145809

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.3-C

Plasmid

#145813

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-PM-miniTurbo-V5_IDG-K

Plasmid

#214454

Purpose

Gateway destination clone of PM-miniTurbo (plasma membrane control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PM-miniTurbo-V5

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAID5.3-N

Plasmid

#145812

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.2-N

Plasmid

#145810

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.4-N

Plasmid

#145814

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAID5.4-C

Plasmid

#145815

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Use

Tags

mAID-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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LTRi

Plasmid

#15913

Depositor

James Collins

Article

Deans et al Cell. 2007 Jul 27. 130(2):363-72.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pUBC-paGFP-Dest

Plasmid

#105107

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Use

Tags

paGFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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