Purpose
pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
Insert
human codon optimized ABE8.20m-nSpG-P2A-EGFP
Use
In vitro transcription; t7 promoter
Tags
BPNLS and BPNLS-3xFLAG-P2A-EGFP
Expression
Mammalian
Mutation
nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
Available since
July 20, 2022
Availability
Academic Institutions and Nonprofits only