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pRRL-MND-hCard11 K69Q-T2A-GFP

Plasmid

#163780

Purpose

Expression of K69Q CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Tags

Expression

Mutation

K69Q

Promoter

MND

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R72K-T2A-GFP

Plasmid

#163785

Purpose

Expression of R72K CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Tags

Expression

Mutation

R72K

Promoter

MND

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 K69R-T2A-GFP

Plasmid

#163782

Purpose

Expression of K69R CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Tags

Expression

Mutation

K69R

Promoter

MND

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRRL-MND-hCard11 R35L-T2A-GFP

Plasmid

#163775

Purpose

Expression of R35L CARD domain variant

Depositor

Richard James

Article

Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

Insert

CARD11 (CARD11 Human)

Use

Lentiviral

Tags

Expression

Mutation

R35L

Promoter

MND

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Use

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-RPS14(WT)-Myc

Plasmid

#122236

Purpose

expresses RPS14 protein with a Myc tag in mammalian cells (hygro resistance)

Depositor

Gerardo Ferbeyre

Article

Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

Insert

RPS14 (RPS14 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1079 - pAAV TH gRNA A EF1a EGFP

Plasmid

#113159

Purpose

An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat TH

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

Plasmid

#113155

Purpose

An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat TH

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6 and hU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

Plasmid

#113157

Purpose

An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

Two gRNAs for rat MANF

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

mU6 and hU6

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEAHISSOarD1

Plasmid

#97067

Purpose

Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus Sso Ard1 protein

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV418/gp25

Plasmid

#97052

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 418/gp25

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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HRas (G12V, E37G)-pcw107

Plasmid

#64639

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

G12V, E37G

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
HRas (G12V, E37G)-pcw107-V5

Plasmid

#64640

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

HRAS (transcript variant 3) (HRAS Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

G12V, E37G

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-LOC442075

Plasmid

#23372

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC442075 (LOC442075 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC390877

Plasmid

#23373

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC390877 (LOC390877 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PANK3

Plasmid

#23384

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK3 (PANK3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC649288

Plasmid

#23391

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC649288 (AK4P6 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC648152

Plasmid

#23513

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC648152 (LOC648152 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

1MM

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only