Addgene

We narrowed to 51,897 results for: cloning

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pFH92

Plasmid

#125820

Purpose

Level0 nScCas9-ABE7.10, wheat/human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9-ABE7.10, wheat/human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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GGE257

Plasmid

#120771

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Xpr2

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFH58

Plasmid

#128184

Purpose

Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDD364

Plasmid

#91831

Purpose

Lox511I-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox511I-SEC-Lox511I

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRATIO2112

Plasmid

#141296

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mScarlet-I and mVenus

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MXS_PGK

Plasmid

#62414

Purpose

MXS_chaining vector with PGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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-24GFP

Plasmid

#25423

Depositor

David J. Goldhamer

Article

Yamamoto et al Mech Dev. . 124(9-10):715-28.

Insert

Myo D 5' flanking sequence (MYOD1 Human)

Use

Reporter construct

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pFH66

Plasmid

#131765

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Use

Tags

PH

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pFH41

Plasmid

#125880

Purpose

Level0 Parsley PcUBI4-2 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Parsley PcUbi4-2 Pol II promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG008

Plasmid

#113982

Purpose

Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Kanamycin/neomycin

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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GGE015

Plasmid

#120770

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Lip2

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRATIO2151

Plasmid

#141300

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFH52

Plasmid

#128181

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(1.3)-L4

Plasmid

#32577

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 1.3kb version

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pFH69

Plasmid

#128189

Purpose

Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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