Addgene

We narrowed to 11,041 results for: cel.2

Showing: 4281 - 4300 of 11041 results

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ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLG327

Plasmid

#128797

Purpose

Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pxyl(s)

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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H52-pUCM-hNGN2-inv

Plasmid

#110492

Purpose

Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm

Depositor

Michael Ward

Inserts

Neurogenin 2 (NEUROG2 Human)
mcherry
rtTA3G

Use

CRISPR, Cre/Lox, and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG, EF-1 alpha, and TRE3G

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Vasa open (K295N) (RR228)

Plasmid

#206435

Purpose

Vasa expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al bioRxiv 2022.03.04.482790

Insert

Vasa (vas Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

K282N

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.1

Plasmid

#208306

Purpose

Expresses ABE-RA4.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.2

Plasmid

#208307

Purpose

Expresses ABE-RA4.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.3

Plasmid

#208308

Purpose

Expresses ABE-RA4.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.4

Plasmid

#208309

Purpose

Expresses ABE-RA4.4in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.4-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.5

Plasmid

#208310

Purpose

Expresses ABE-RA4.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.5-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.6

Plasmid

#208311

Purpose

Expresses ABE-RA4.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.6-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEYFP(Q69K)-mEGFP

Plasmid

#190751

Purpose

Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2, mEGFP, and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-sfGFP

Plasmid

#163757

Purpose

Expresses Streptomyces codon-optimised sfGFP

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Mutation

Promoter

SP44

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pDMS151A_Tet-On-3G

Plasmid

#191577

Purpose

ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

Tet-On(R) 3G

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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MI-Luciferase-IRES-mCherry

Plasmid

#75020

Purpose

Encodes the expression of Luciferase and mCherry

Depositor

Xiaoping Sun

Article

Mallampati et al Blood. 2014 Jun 26;123(26):4064-76. doi:

Inserts

firefly Luciferase
mCherry

Use

Luciferase and Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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3120 pSFFV-neo Bcl-XL

Plasmid

#8749

Depositor

Stanley Korsmeyer

Article

Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

Insert

Bcl-xl (BCL2L1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pSG5-SUV39H2-FL

Plasmid

#89135

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regions

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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