Addgene

Showing: 4281 - 4300 of 148509 results

Results per page:

Sort results by:

pTRIP-CMV-mCherry-53BP1

Plasmid

#127658

Purpose

Over-express mCherry-53BP1 fragment to monitor 53BP1 foci.

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

53BP1 (TP53BP1 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Nucleotides 3658-5133 of TP53BP1 (NM_005657.2) fu…

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hSTARR-seq_SCP1 vector_blocking 4

Plasmid

#99319

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCambia1300-PacI

Plasmid

#44183

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_L_EBOV

Plasmid

#103052

Purpose

Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

L gene of Ebola virus (L Zaire ebolavirus, Mayinga)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose-inducible pBAD

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mEmerald-CD81-10

Plasmid

#54031

Purpose

Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

Insert

CD81 (CD81 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
AAV.CAG.GLPLight1

Plasmid

#187467

Purpose

Expresses GLPLight1 in mammalian cells

Depositor

Tommaso Patriarchi

Article

Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

Insert

GLPLight1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

CAG

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS_VP30_EBOV

Plasmid

#103051

Purpose

Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Cerulean

Plasmid

#15214

Depositor

Dave Piston

Article

Rizzo et al Nat Biotechnol. 2004 Apr . 22(4):445-9.

Insert

monomeric Cerulean

Use

Tags

Expression

Mammalian

Mutation

A206K

Promoter

Available since

June 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
XLone-PE2

Plasmid

#136463

Purpose

Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

Depositor

Xiaojun Lian

Insert

PE2

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FlagMBNL3

Plasmid

#96901

Purpose

expression of Flag-tagged human MBNL3

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

human MBNL3 cDNA (MBNL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CMV/TO Zeo DEST (644-1)

Plasmid

#17294

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Zeo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

Plasmid

#121944

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

U6 promoters

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE8r

Plasmid

#208294

Purpose

Expresses ABE8r in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8r-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-Geminin(1-110)

Plasmid

#83915

Purpose

Fluorescent probe for M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-ITSN1

Plasmid

#129614

Purpose

RhoGEF

Depositor

Dorus Gadella

Article

Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

Insert

ITSN1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT/TO/HIS/FLAG/HA-MYBBP1A

Plasmid

#38084

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

MYBBP1A MYB binding protein (P160) 1a (MYBBP1A Human)

Use

Tags

HIS/FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcg 2.0-BFP-circ-arRNA backbone

Plasmid

#213965

Purpose

pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge