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Plasmid#221260PurposeExpression of IRF3 in mammalian cells by retroviral transductionDepositorInsertIRF3 (Irf3 Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-UDP1.0-IRES-mCherry-CAAX
Plasmid#220850PurposeCMV promoter drives the expression of an extracellular UDP sensor (UDP1.0) and a membrane localized mCherryDepositorInsertUDP1.0
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCF4 in pENTR
Plasmid#216569Purposegateway cloning donor vector containing TCF4DepositorInsertTCF4 (TCF4 Human)
UseGateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-tadDE-PcrA M6-UGI
Plasmid#221236PurposeExpress HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.DepositorInserttadDE-PcrA M6-UGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCas9-T2A-mApple
Plasmid#193780PurposeTricistronic expression of Cas9-mApple-BSD in mammalian cellsDepositorInsertmApple
UseLentiviralTagsExpressionMammalianMutationPromoterIn frame with Cas9Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS140_6xHis-EGFP-I3-01
Plasmid#211889PurposeBacterial expression of I3-01DepositorInsertI3-01
UseTagsExpressionBacterialMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
MECP2 - R133C in pENTR
Plasmid#216594Purposegateway cloning donor vector containing MECP2 ASD mutationDepositorInsertMECP2 (MECP2 Human)
UseGateway entry plasmidTagsExpressionMutationR133CPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA_OROV_NSs
Plasmid#208939PurposeExpresses C-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 66 bp from 5' end and deleted 415 b…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHA_OROV_NSm
Plasmid#208940PurposeExpresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cellsDepositorInsertNSm
UseTagsHAExpressionMammalianMutationDeleted 949 bp from 5' end (Gn), deleted 29…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NSm
Plasmid#208935PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cellsDepositorInsertNSm
UseTagsHAExpressionMammalianMutationDeleted 949 bp from 5' end (Gn), deleted 29…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CRISPR-Puro
Plasmid#208646PurposepcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.DepositorTypeEmpty backboneUseCRISPRTagsClawed frog nucleoplasmin NLS, Flag, Puromycin re…ExpressionMammalianMutationPromoterCMV and U6 in tandemAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCXMG
Plasmid#204409PurposeExpression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI siteDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromotertacAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
plYJP067-Ptet
Plasmid#202299PurposePTet-YFP plasmid for LPattB2 integrationDepositorInsertPtet-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2601
Plasmid#144133PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertPOU6F2 (POU6F2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pShuttle
Plasmid#192871PurposeGateway compatible vector with BsaI cloning siteDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCosSAD1AprR
Plasmid#190786PurposeAn E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombinationDepositorInsertsupstream region of the aur1 cluster for auricin
ampramycin resistance cassette
downstream region of the aur1 cluster for auricin
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro Cas13d-eGFP U6 SOX17 g2
Plasmid#155188PurposePiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2DepositorInsertCas13d SOX17 gRNA2
UsePiggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-ZNF804B
Plasmid#88756PurposeDonor Vector containing ZNF804B transcription factor, part of the Human TFome CollectionDepositorInsertZNF804B (ZNF804B Human)
UseGateway donor vectorTagsExpressionMutationLast nucleotide of stop codon removed to allow fo…PromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
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RCAS GH6 (CC#152)
Plasmid#15137DepositorInsertGH6 (HMX1 Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS SOHo1 (CC#99)
Plasmid#15132DepositorInsertSOHo1 (SOHO-1 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W
Plasmid#67981PurposeCas9 activity reporter (control) with mCherry and GFPDepositorInsertU6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPREPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PE7_AAVS1_knockin_HDR_donor
Plasmid#223001PurposeHDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_armDepositorInsertPE7
UseHdr donorTagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationPromoterEF1αAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA559
Plasmid#186235PurposepTet-LbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECJ3
Plasmid#75465PurposeToggle switch plasmid containing lacI-pdt3DepositorInsertlacI-pdt3
UseTagsExpressionBacterialMutationPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Cas9_Blast
Plasmid#125592PurposeLentiviral expression of spCas9 with blasticidin resistance geneDepositorInsertCas9
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-2A-EGFP
Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotertTRE, hPGKAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP16
Plasmid#22595DepositorInsertUSP16 (USP16 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only