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Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEBB-BT
Plasmid#36295DepositorTypeEmpty backboneUseTagsBT-tag (peptide sequence from P.freudenreichii tr…ExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pGBW-m4046933
Plasmid#145592PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pETM41-EcPPXc
Plasmid#38329DepositorInsertE. coli polyphosphatase (ppx Escherichia coli)
UseTagsHis, MBP, and TEVExpressionBacterialMutationC-terminus of E. coli polyphosphatase (described …PromoterT7Available sinceAug. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046901
Plasmid#145725PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7a proteinDepositorInsertSARS-CoV-2 ORF7a protein (ORF7a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH-HT_CtCHAD
Plasmid#127785PurposeExpresses CtCHAD in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain)PromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Rca_Cas13a_WT
Plasmid#91870PurposeBacterial expression for Cas13aDepositorInsertRcaCas13a (RcaC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046940
Plasmid#149261PurposeBacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046907
Plasmid#149262PurposeBacterial expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMH-HT_SsCHAD
Plasmid#127783PurposeExpresses SsCHAD in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
UseTags6xHis tag + TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
GRLF1
Plasmid#36195PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRLF1 (Codon Devices Synthesized) (ARHGAP35 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationContains the Ras homolog domain only (amino acid …PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2CT-His-MBP-Cam_Cas13a_WT
Plasmid#91869PurposeBacterial expression for Cas13aDepositorInsertCamCas13a (CamC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Ere_Cas13a_WT
Plasmid#91868PurposeBacterial expression for Cas13aDepositorInsertEreCas13a (EreC2c2) WT
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046854
Plasmid#145759PurposeBacterial Expression plasmid for SARS-CoV-2 leader proteinDepositorInsertSARS-CoV-2 leader protein (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046832
Plasmid#145691PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046841
Plasmid#145578PurposeBacterial Expression plasmid for SARS-CoV-2 helicaseDepositorInsertSARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046953
Plasmid#145764PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
p2CT-His-MBP-Lbu_C2c2_K1087A
Plasmid#91864PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1087A
UseTagsHis6-MBP-TEV siteExpressionBacterialMutationK1087APromoterAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only