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hFLN29 L175-end/pCMV-14-FLAG

Plasmid

#73711

Purpose

Express human TRAFD1 (FLN29) from L175 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 174 aa from full lenght TRAFD1

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 G117-end/pCMV-14-FLAG

Plasmid

#73710

Purpose

Express human TRAFD1 (FLN29) from G117 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 116 aa from full lenght TRAFD1

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29 C60-end/pCMV-14-FLAG

Plasmid

#73709

Purpose

Express human TRAFD1 (FLN29) fromC60 to end in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of first 59 aa from full lenght TRAFD1

Promoter

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
hFLN29-C/pCMV-14-FLAG (Q391-END)

Plasmid

#73590

Purpose

Express C-terminal part (Q391- end) of human TRAFD1(FLN29) in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

TRAFD1 (TRAFD1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP-GFP

Plasmid

#73579

Purpose

Lentivirus expression of mOC-STAMP

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT

Plasmid

#61218

Purpose

Lentivirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV/TO

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 RNAiR2 short

Plasmid

#51596

Purpose

expresses RNAiR myc-tagged rat Rem2 (short version, missing 69 AA at N terminus)

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

silent mutations at shRNA 1 and 2 recognition sit…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-myc-Rem2 R236A/L263A

Plasmid

#51589

Purpose

expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R236A and L263A; silent mutations at shRNA 1 and …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A/S334A

Plasmid

#51588

Purpose

expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S69A and S334A; silent mutations at shRNA 1 and 2…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLA CMV N-Flag RALB R47

Plasmid

#50991

Purpose

Expresses RALB-R47 in mammalian cells

Depositor

Anna Sablina

Article

Simicek et al Nat Cell Biol. 2013 Oct;15(10):1220-30.

Insert

RALB (RALB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

K47R

Promoter

CMV

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127A/G385V

Plasmid

#33072

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, G385V

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-S127/D383A

Plasmid

#33078

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

S127A, D383A

Promoter

CMV

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pCMV Ub Lex VP16 (F442A) HA (P#1711)

Plasmid

#14596

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA, Ubiquitin, and VP16 (F442A)

Expression

Mammalian

Mutation

The VP16 sequence contains an F442A mutation. The…

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pLenti CMV Hygro DHFR.dn-cHSF1

Plasmid

#134731

Purpose

Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Lentiviral

Tags

DHFR

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV

Available since

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

Plasmid

#115141

Purpose

CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Orf2mor anti-CRISPR protein

Use

Tags

NLS(SV40)

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-ECL-3TM-tevs-KKMP V3

Plasmid

#179640

Purpose

TEVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpRY-P2A-EGFP (KAC1069)

Plasmid

#185912

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpRY-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CMV and T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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