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Showing: 4221 - 4240 of 150501 results

  2. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  3. mRFP-Rab7

    Plasmid
    #14436
    Purpose
    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Article
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Insert
    Rab 7 (RAB7A Canis familialis)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    3 silent mutations near the C-terminus
    Promoter
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    14436-plasmid-map-sequence-id-229535

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  5. pDONRp5-p2 SspB-mCherry-CShroom3

    Plasmid
    #170977
    Purpose
    C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.
    Depositor
    Miki Ebisuya
    Article
    Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:
    Insert
    CShroom3 (Shroom3 Mouse)
    Use
    Gateway cloning
    Tags
    SspB and mCherry
    Expression
    Mutation
    Isoform X2, deleted aminoacids 1 - 1388
    Promoter
    Available since
    July 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170977-plasmid-map-sequence-id-336791

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  9. TFORF0757

    Plasmid
    #143074
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NOTCH1 (NOTCH1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143074-plasmid-map-sequence-id-399050

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  10. pDGB3alpha2_35S:P19:Tnos (GB1203)

    Plasmid
    #68214
    Purpose
    Transcriptional unit for Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression driven by the 35S promoter
    Depositor
    Diego Orzaez
    Article
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    P19 silencing suppressor
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68214-plasmid-map-sequence-id-119066

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  11. pDRM55 tet-AR(1-707)

    Plasmid
    #183503
    Purpose
    Lentiviral vector expressing a doxycycline-inducible constitutively active truncated androgen receptor (wild type AR)
    Depositor
    Wayne Tilley
    Article
    Hickey et al Nat Med. 2021 Feb;27(2):310-320. doi: 10
    Insert
    Androgen receptor (AR Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Constitutively active truncated AR; spans AR 1-70…
    Promoter
    CMV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183503-plasmid-map-sequence-id-362517

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  18. TFORF2237

    Plasmid
    #141983
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA4 (GATA4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    141983-plasmid-map-sequence-id-386554

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Showing: 4221 - 4240 of 150501 results