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Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Bighead S-HA
Plasmid#172485Purposefor synthesis of mRNA for injection into xenopus laevis embryosDepositorInsertBighead.S
UseIn vitro mrna synthesisTagsHA and S-HAExpressionMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-xFAM3b.L-HA
Plasmid#172852PurposemRNA synthesis for injection into embryosDepositorInsertxFAM3B.L (fam3b.L Frog)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-NbBCP1bPro
Plasmid#159417PurposeNicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertNbBCP1b Pro
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 WAPL gRNA
Plasmid#165595PurposeInsertion of WAPL degromDepositorInsertWAPL (Wapl Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
px330 BRM gRNA
Plasmid#165586PurposeInsertion of BRM degronDepositorInsertBRM (Smarca4 Mouse)
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
DT040A
Plasmid#159401PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli hybrid J23117-J23106 promoterDepositorInsertpr.J23117/J23106-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialMutationPromoterhybrid J23117-J23106 promoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfKitlgb
Plasmid#140293PurposeExpress soluble His-tagged zebrafish Kitlgb in insect cellsDepositorInsertKitlgb (kitlgb Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM28
Plasmid#154165PurposeAedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virusDepositorInsertRel1A
UseTagsFLAGExpressionInsectMutationPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM108
Plasmid#134160PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_KMLL E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationKMLL, E. coli codon optimized, CRISPR/Cas9 harden…PromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM107
Plasmid#134159PurposeRetroviral plasmid expressing GFP - Nde1DepositorInsertNde1_SSSC E. coli codon optimized (CRISPR/Cas9 hardened) (NDE1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationSSSC, E. coli codon optimized, CRISPR/Cas9 harden…PromoterAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-ATG1(mut)(406)
Plasmid#122069PurposeExpressing a mutant ATG1DepositorInsertATG1 (ATG1 Budding Yeast)
UseTagsExpressionYeastMutationCodon changes without changing the amino acid seq…PromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A)(405)
Plasmid#122065PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(S226A,T228A)(405)
Plasmid#122066PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
UseTagsPAExpressionYeastMutationSerine 226 to alanine; Threonine 228 to alaninePromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC/mCitrine/erg3-mCherry
Plasmid#117993PurposeCo-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoterDepositorInserterg3-mCherry
UsePlant/bacteria binary vectorTagsExpressionPlantMutationPromoterGal10Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only