Addgene

We narrowed to 13,534 results for: pET

Showing: 4201 - 4220 of 13534 results

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pP450cam

Plasmid

#85085

Purpose

Expresses P450cam (CYP101A1) in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450cam

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pPdR

Plasmid

#85083

Purpose

Expresses putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pJK_proB_eGFP

Plasmid

#59845

Purpose

Constitutively expresses eGFP with proB promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proB

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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Talin Head

Plasmid

#186140

Purpose

For expression of recombinant Talin head domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

TLN1 (TLN1 Human)

Use

Tags

His6 (tev cleavage)

Expression

Bacterial

Mutation

Promoter

Lac

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_eGFP

Plasmid

#59853

Purpose

Constitutively expresses eGFP with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK1

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCB

Plasmid

#127471

Purpose

coding sequence for synthetic zinc finger ZF-BCB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCB synthetic transcription factor

Use

Cell-free transcription–translation

Tags

Expression

Mutation

Promoter

T7

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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K560GFP_17NL

Plasmid

#129767

Purpose

Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays

Depositor

William Hancock

Insert

KHC (Khc Fly)

Use

Tags

GFP and His6

Expression

Bacterial

Mutation

DAL insert before A345

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_proK6_eGFP

Plasmid

#59850

Purpose

Constitutively expresses eGFP with proK6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK17_amiE

Plasmid

#59837

Purpose

Constitutively expresses AmiE with proK17 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

ALIPHATIC AMIDASE (amiE )

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

proK17

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCri-8a-Strep

Plasmid

#61340

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Use

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pJK_pro6_eGFP

Plasmid

#59851

Purpose

Constitutively expresses eGFP with pro6 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

pro6

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pJK_proK14_eGFP

Plasmid

#59848

Purpose

Constitutively expresses eGFP with proK14 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Use

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK14

Available since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pQA-LP574

Plasmid

#222953

Purpose

Expresses R120A LoaP

Depositor

Adrian Ferré-D'Amaré

Article

Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

Insert

antiterminator protein LoaP

Use

Tags

10xHIS-SUMO

Expression

Bacterial

Mutation

mutated Arginine 120 to Alanine

Promoter

T7

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM284

Plasmid

#226122

Purpose

Expression of cdc48 E588Q

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-3C-Cdc48 E588Q (CDC48 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

E588Q

Promoter

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM290

Plasmid

#226127

Purpose

Expression of His6-GST-3C-Npl4

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM289

Plasmid

#226126

Purpose

Expression of Npl4 D460K/Y461A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 D460K/Y461A (NPL4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

D460K/Y461A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM358

Plasmid

#226138

Purpose

Expression of His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-mTurqoise2-intein-CBD

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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paM291

Plasmid

#226128

Purpose

Expression of His6-GST-3C-Npl4 T255A/T571A

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-GST-3C-Npl4 T255A/T571A (HIS6 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

T255A/T571A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM288

Plasmid

#226125

Purpose

Expression of Npl4 W252A/R253E

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Npl4 W252A/R253E (NPL4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

W252A/R253E

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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