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pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL50015

Plasmid

#50318

Purpose

Please note- this plasmid is currently not distributed in the MoClo Plant Parts Kit (# 1000000047)

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mNEON (Branchistoma lanceolatum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSCW02

Plasmid

#72301

Purpose

Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Type

Empty backbone

Use

Derivative of puc19

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pICSL50016

Plasmid

#50315

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.

Use

Plant expression

Tags

Expression

Mutation

Codon-optimised for A. thaliana. BsaI/BbsI sites …

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pre_IV_RFP_1317_v1

Plasmid

#126863

Purpose

1317 b scaffold based on pSB1A3 backbone with RFP gene as insert

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_RFP_1317_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E3E4 (630–1244)

Plasmid

#132628

Purpose

expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (630–1244)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAcGP67A human LRP6 E1E4 (20–1244)

Plasmid

#132629

Purpose

expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

Depositor

Wenqing Xu

Article

Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

Insert

LRP6 (20–1244)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_345_Citrine

Plasmid

#130592

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_345

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_10E2_Cherry

Plasmid

#130590

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_10E2

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_87_Cerulean

Plasmid

#130586

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_87

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Sox10_enh_85_Citrine

Plasmid

#130585

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox10_enh_85

Use

Enhancer reporter

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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tGFP

Plasmid

#26864

Depositor

Linzhao Cheng

Article

Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

Insert

Truncated GFP-IRES-Puro

Use

Tags

Expression

Bacterial

Mutation

Deletion of nt 1-36

Promoter

Available since

Nov. 19, 2010

Availability

Academic Institutions and Nonprofits only

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JP1520 PIK3CA H1047R HA

Plasmid

#14572

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA H1047R (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
pICH51277

Plasmid

#50268

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF002

Plasmid

#48843

Purpose

Provides a BASTA resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::BastaR::t35S

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

internal BsaI recognition site in 35S promoter re…

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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tetO7_Ef1a_SOX2

Plasmid

#18919

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

SOX2 (SOX2 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pICSL70002

Plasmid

#50333

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLNCX2-lifeact-mAPPLE

Plasmid

#89820

Purpose

mAPPLE labeled fluorescent actin

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

lifeact-mAPPLE

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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