Addgene

We narrowed to 51,897 results for: cloning

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GGE085

Plasmid

#120738

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_Tef

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-hTRIM9

Plasmid

#51032

Purpose

entry vector for cloning human TRIM9

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

TRIM9 (TRIM9 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRATIO2214

Plasmid

#141303

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Use

Tags

mCerulean and mScarlet-I

Expression

Plant

Mutation

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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GGE001

Plasmid

#120740

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_PGM

Use

Cloning/assembly plasmid

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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p663-UBC-V5-BRSK1_IDG-K

Plasmid

#135270

Purpose

Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

V5-BRSK1 (BRSK1 Human)

Use

Lentiviral; Gateway destination

Tags

V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T

Plasmid

#64954

Purpose

mini-Tn7 base vector with transcriptional terminator

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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p3E LAP2b

Plasmid

#105978

Purpose

3' element for Gateway cloning, lamina-associated polypeptide 2

Depositor

Caren Norden

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

LAP2b

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMK-N. tabacum trnI/trnA

Plasmid

#123579

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

N. tabacum trnI/trnA integration site

Use

Chloroplast transformation vector

Tags

Expression

Plant

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

Plasmid

#164263

Purpose

Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

Depositor

Joseph Peters

Article

Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_BiTet

Plasmid

#62423

Purpose

MXS_chaining vector with BiTet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bidirectional tetracycline inducible promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate2

Plasmid

#25941

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Jun 1. ():.

Insert

Use

Tags

VP16 Fusion Protein

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate2

Plasmid

#25947

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Jun 1. ():.

Insert

Use

Tags

GAL4 Fusion Protein

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pGolden-AAV

Plasmid

#51424

Purpose

Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

lacZ

Use

AAV

Tags

Expression

Mutation

Promoter

lacZ

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP02

Plasmid

#194351

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBSDONR P4r-P2

Plasmid

#121527

Purpose

Gateway Entry Vector for Multisite Gateway Cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-Kir2.1-mCherry-L2

Plasmid

#32669

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Kir2.1-mCherry

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 22, 2012

Availability

Academic Institutions and Nonprofits only

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pKER-mKOk

Plasmid

#85975

Purpose

Expresses mKOk from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mKOk

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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