Skip to main content
Addgene
Showing: 8401 - 8440 of 156661 results

  3. TFORF0731

    Plasmid
    #142201
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PROX1 (PROX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142201-plasmid-map-sequence-id-397649

    Enlarge

  4. pCDH-CB-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73039
    Purpose
    Express Nluc, copGFP dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken beta-actin + CMV enhancer and chicken bet…
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73039-plasmid-map-sequence-id-136043

    Enlarge

  7. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

    Enlarge

  8. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

    Enlarge

  10. pBABE GFP

    Plasmid
    #10668
    Purpose
    Empty backbone for retroviral gene expression. Select recipient cells using GFP.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 11, 2005
    Availability
    Academic Institutions and Nonprofits only
    10668-plasmid-map-sequence-id-106108

    Enlarge

  11. AiP13798 - pAAV-DLX2.0-minBG-SYFP2-P2A-mScarlet-10aa-H2B-WPRE3-BGHpA (Alias: CN3798)

    Plasmid
    #229935
    Purpose
    DLX2.0 is an enhancer sequence, designed to drive cytosolic SYFP2 and nuclear mScarlet expression in forebrain GABAergic neurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.
    Insert
    SYFP2-P2A-mScarlet-10aa-H2B
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    229935-plasmid-map-sequence-id-460618

    Enlarge

  13. pDONR221-MTCH2_STOP

    Plasmid
    #161120
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    Giulio Superti-Furga, RESOLUTE Consortium
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    MTCH2 (MTCH2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161120-plasmid-map-sequence-id-360077

    Enlarge

  14. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid
    #188899
    Purpose
    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Jonathan Weissman, Marco Jost
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Zim3-dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188899-plasmid-map-sequence-id-374831

    Enlarge

  15. pCDNA3- c-terminal 6xMyc

    Plasmid
    #128023
    Purpose
    Mammalian expression vector with C terminal 6xMyc tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    6xMyc tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    128023-plasmid-map-sequence-id-254748

    Enlarge

  18. pOPINB-HOIL-1 full-length (1–510)

    Plasmid
    #193858
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Codon optimised for expression in E. coli
    Promoter
    T7
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193858-plasmid-map-sequence-id-379617

    Enlarge

  22. pEGB2_alpha2 P35S:CUP2:GAL4AD:T35S (GB1039)

    Plasmid
    #160607
    Purpose
    TU for the constitutive expression of the transcriptional activator protein CUP2 in C-terminal fusion with GAL4 activation domain (GAL4AD). Binds to specific DNA operator in the presence of copper ions and promotes transcription.
    Depositor
    Diego Orzaez
    Insert
    CUP2Gal4AD
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    P35S
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160607-plasmid-map-sequence-id-322043

    Enlarge

  26. pLS-mP-Luc

    Plasmid
    #106253
    Purpose
    Luciferase lentivirus based enhancer assay vector
    Depositor
    Nadav Ahituv
    Insert
    Luciferase
    Use
    Lentiviral and Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Derived from pGL4.23
    Available since
    May 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    106253-plasmid-map-sequence-id-208513

    Enlarge

  29. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

    Enlarge

  35. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

    Enlarge

  39. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid
    #221566
    Purpose
    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Article
    Ruffolo et al bioRxiv 2024.04.22.590591
    Insert
    OpenCRISPR-1 sgRNA 16nt stem loop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221566-plasmid-map-sequence-id-437904

    Enlarge

Showing: 8401 - 8440 of 156661 results