-
Plasmid#126854Purpose9072 b scaffold from compatibility group 3, made by method IIDepositorInsertII_CS3_9072_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-SaDAH
Plasmid#140128PurposeExpresses SaDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Sinomenium acutum
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ143-pcDNA3-BvCas12b
Plasmid#122445PurposeExpresses BvCas12b in mammalian cellsDepositorInsertCMV-NLS-BvCas12b-NLS-3xHA
UseTags3XHAExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ147-BvCas12b-sgRNA-scaffold
Plasmid#122447PurposeExpresses sgRNA for BvCas12b in mammalian cellsDepositorInsertU6-BvCas12b-sgRNA-scaffold, CMV-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_zyxin
Plasmid#83034PurposeExpresses sfCherry2(11) tagged zyxin (human) in mammalian cellsDepositorInsertsfCherry2(11)_zyxin
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH87633
Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pICSL70008
Plasmid#50336DepositorInsertpFAST-R selection cassette (monomeric tagRFP from E. quadricolor fused to the coding sequence of OLE1 (A. thaliana)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCV-HF
Plasmid#32057DepositorInsertMCV-HF genome
UseTagsExpressionMutationPromoternone from vector. Insert contains native promoter…Available sinceOct. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7560_v1
Plasmid#126853Purpose7560 b scaffold from compatibility group 3, made by method IIDepositorInsertII_CS3_7560_v1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox9_enh_283_Citrine
Plasmid#130591Purposeenhancer reporterDepositorInsertSox9_enh_283
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_sfCherry2(11)_Keratin
Plasmid#82604PurposeExpresses sfCherry2(11) tagged keratin in mammalian cellsDepositorInsertsfCherry2(11)_Keratin
UseTagsExpressionMammalianMutationchanged Gly12 to Ala in sfCherry11+PromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSCW01
Plasmid#72300PurposeStarting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.DepositorTypeEmpty backboneUseDerivative of puc19TagsExpressionBacterialMutationPromoterlacAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
p70 delta2-46 delta CT104
Plasmid#1898DepositorInsertp70 alpha1 S6 kinase (Rps6kb1 Rat)
UseTagsHAExpressionMammalianMutationdelta2-46, deltaCT104 (deletion of aa 2-46 and am…PromoterAvailable sinceMarch 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMYs-IRES-mitoRFP
Plasmid#121996PurposeExpression of mitoRFP in mammalian cellsDepositorInsertIRES-mitoRFP
UseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
px458_Conc3
Plasmid#134450PurposeEmpty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFPDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E2 (20–629)
Plasmid#132627Purposeexpresses human LRP6 E1E2 (20–629) in Hi5 insect cellDepositorInsertLRP6 (20–629)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Snai2_enh_13_Citrine
Plasmid#130574Purposeenhancer reporterDepositorInsertSnai2_enh_13
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK_Sox10_enh_84_Citrine
Plasmid#130584Purposeenhancer reporterDepositorInsertSox10_enh_84
UseEnhancer reporterTagsExpressionMutationPromoterthymidine kinase minimal promoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC155-pCR8-sgExpression
Plasmid#49045PurposeU6-driven sgRNA expressing cassette on a gateway donor vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRKO
Plasmid#49456PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptionsDepositorInsertloxP-kanMX-loxP cassette
UseCre/LoxTagsExpressionBacterialMutationPromoternoneAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only