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pDRF1 Cyterm-ymStayGold

Plasmid

#219855

Purpose

expression of Cyterm-ymStayGoldd in budding yeast

Depositor

Bas Teusink

Insert

Cyterm-ymStayGold

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

Tags

StayGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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VC-Y705F+K49R-STAT3

Plasmid

#203721

Purpose

Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

Insert

STAT3 tagged with VC (aa 158-238) (STAT3 Human)

Use

Tags

VC (aa 158-238) for bimolecular fluorescence comp…

Expression

Mammalian

Mutation

Y705F+K49R substitutions

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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VC-Y705F+K685R-STAT3

Plasmid

#203722

Purpose

Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

Insert

STAT3 tagged with VC (aa 158-238) (STAT3 Human)

Use

Tags

VC (aa 158-238) for bimolecular fluorescence comp…

Expression

Mammalian

Mutation

Y705F+K685R substitutions

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-sTagRFP-Zeo

Plasmid

#207734

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Zeo-sTagRFP

Plasmid

#207701

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-sTagRFP-Blast

Plasmid

#207712

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-N-Puro-sTagRFP

Plasmid

#207690

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-sTagRFP

Plasmid

#207679

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Ac5-STABLE1-blast

Plasmid

#208033

Purpose

Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_STAT1-2_Mutant

Plasmid

#194189

Purpose

Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated STAT1/2 site, Region 653-673nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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sTag-BFP-6xHIS

Plasmid

#186905

Purpose

Bacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tag

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-6xHIS

Use

Tags

tagBFP, 6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-STAT2

Plasmid

#183931

Purpose

Optogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#1/Cre

Plasmid

#173659

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgStag2#2/Cre

Plasmid

#173660

Purpose

Expresses a Stag2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Stag2 (Stag2 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBS21

Plasmid

#171885

Purpose

Promoter SPLc19 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc21

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc19-RBSc249

Plasmid

#171904

Purpose

Promoter SPLc19 and RBSc249 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc19 and RBSc249

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pStA0::SPLc25-RBSc123

Plasmid

#171900

Purpose

Promoter SPLc25 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc25 and RBSc123

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc45-RBSc21

Plasmid

#171887

Purpose

Promoter SPLc45 and RBSc21 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc21

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pStA0::SPLc45-RBSc123

Plasmid

#171901

Purpose

Promoter SPLc45 and RBSc123 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc45 and RBSc123

Use

Synthetic Biology

Tags

Expression

Mutation

N/A

Promoter

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge