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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-CALPAIN-7-mCherry, F98D

Plasmid

#180653

Purpose

Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant to: GCACCCAUACCUUUACAUU

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KL1 MIT, residues 20-140

Plasmid

#180623

Purpose

Bacterial expression for RPS6KL1 MIT domain, residues 20-140. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-32.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KL1 (RPS6KL1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 20-140

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-SNX15, residues 263-342

Plasmid

#180624

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-GST tag. Internal ID:WISP21-130.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 263-342

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SNX15 MIT residues 263-342

Plasmid

#180625

Purpose

Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SNX15 (SNX15 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 263-342

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , F124D, residues 108-200

Plasmid

#180629

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, F124D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , L177D, residues 108-200

Plasmid

#180634

Purpose

Bacterial expression for SPASTIN MIT, residues 108-200. Has L177D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-13.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 108-200, L177D

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP8, residues 1-147

Plasmid

#180636

Purpose

Bacterial expression for USP8 MIT domain, residues 1-147. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-86.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP8 (USP8 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-147

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 USP54, residues 731-878

Plasmid

#180637

Purpose

Bacterial expression for USP54 MIT domain, residues 731-878. Has N-terminal HIS-SUMO tag. Internal ID:WISP20-85

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

USP54 (USP54 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, V18D

Plasmid

#180612

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has V18D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-26.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, V18D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 CALPAIN-7 residues 1-165, F98D

Plasmid

#180613

Purpose

Bacterial expression for CALPAIN-7 MIT domains, residues 1-165. Has F98D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-27.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CALPAIN-7

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-165, F98D mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP NRBF2 MIT residues 1-111

Plasmid

#180621

Purpose

Bacterial expression for NRBF2 MIT domain, residues 1-111. Has N-terminal HIS-GST. Internal ID: WISP20-88.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

NRBF2 (NRBF2 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-111

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-RHD272-472-GFP

Plasmid

#186606

Purpose

Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Met-Gly-Ser-Lys peptide added at N-terminus for c…

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-RHD272-472-mNeon

Plasmid

#186605

Purpose

Encodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Met-Gly-Ser-Lys peptide added at N-terminus for c…

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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DsRed2-M11-92

Plasmid

#134467

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-M11-92

Plasmid

#134465

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-MED1LCD

Plasmid

#204404

Purpose

E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

MED1(947-1581) (MED1 Human)

Use

Tags

6x-His; mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Use

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB6

Plasmid

#63107

Purpose

Expression of HSPB6 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb6 (HSPB6 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Use

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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