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Plasmid#86083PurposeA lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.DepositorInsertcontrol shRNA
UseTagsExpressionMutationPromoterU6Available sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P06
Plasmid#120017PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with sfGFPDepositorInsertsfGFP
UsePcr templateTagssfGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-pGK:GFP
Plasmid#68486PurposeRetrovirus expressing GFP driven by pGK promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and pGKAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMOX-mNG::LacI-V3
Plasmid#210224PurposeIntegrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.DepositorInsertmNeongreen Lac repressor fusion protein
UseTrypanosoma brucei expressionTagsExpressionMutationPromoterPol II readthrough transcription after integrationAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
SHC003BSD
Plasmid#133300PurposeThe puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCL1032
Plasmid#217962PurposeMammalian expression of NASP ('s' isofrom) fused at its N-terminus to eGFP with a TEV protease site separating the two. Puromycin resistance is expressed from a downstream IRES.DepositorInsertNASP (NASP Human)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P31
Plasmid#120042PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5DepositorInsertV5
UsePcr templateTagsV5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-UBC:GFP
Plasmid#68482PurposeRetrovirus expressing GFP driven by UBC promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and UBCAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P02
Plasmid#120013PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-SV40:GFP
Plasmid#68483PurposeRetrovirus expressing GFP driven by SV40 promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterLTR and SV40Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAPM D4 BsmBI Myd88 stuffer
Plasmid#128505PurposepAPM knockdown vector with Myd88 stuffer sequenceDepositorInsertPuromycin Resistance
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P14
Plasmid#120025PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5DepositorInsertmNeptune2.5
UsePcr templateTagsmNeptune2.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P10
Plasmid#120021PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOkDepositorInsertmKOk
UsePcr templateTagsmKOkExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P15
Plasmid#120026PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670DepositorInsertmiRFP670
UsePcr templateTagsmiRFP670ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P24
Plasmid#120035PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAPDepositorInsertTAP
UsePcr templateTagsTAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro-EFS:GFP
Plasmid#68484PurposeRetrovirus expressing GFP driven by EFS promoterDepositorInsertsPuromycin Resistance
GFP
UseRetroviralTagsExpressionMammalianMutationPromoterEFS and LTRAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P18
Plasmid#120029PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5DepositorInsertmKeima8.5
UsePcr templateTagsmKeima8.5ExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P17
Plasmid#120028PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4bDepositorInsertmEos4b
UsePcr templateTagsmEos4bExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P01
Plasmid#120012PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxBFPDepositorInsertmoxBFP
UsePcr templateTagsmoxBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only