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Showing: 401 - 420 of 2405 results

  5. pEF-pIgH-ESCargo*

    Plasmid
    #140159
    Purpose
    Expresses a regulatable secretory cargo (without an ER export signal) for mammalian cells
    Depositor
    Benjamin Glick
    Article
    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.
    Insert
    piGH-DsRed-Express2-FKBP(LV)(C22V)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140159-plasmid-map-sequence-id-275932

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  8. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  14. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Expression
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

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  20. pUBC_HA_PP7-mCherry

    Plasmid
    #107605
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Article
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Insert
    PP7-mCherry
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    hUBC
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    107605-plasmid-map-sequence-id-207484

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Showing: 401 - 420 of 2405 results