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Showing: 401 - 420 of 5880 results

  1. TMD18

    Plasmid
    #163673
    Purpose
    Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163673-plasmid-map-sequence-id-322677

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  2. TMD19

    Plasmid
    #163674
    Purpose
    Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    For this partial length ST chimera, two restricti…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163674-plasmid-map-sequence-id-322678

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  3. FKBP-ST*-GFP

    Plasmid
    #163669
    Purpose
    Expresses FKBP tagged partial length ST in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    Use
    Tags
    FK506 binding protein (FKBP) and GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the first 113 amino acids o…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163669-plasmid-map-sequence-id-322815

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  4. pTF101.1gw3

    Plasmid
    #172181
    Purpose
    A binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.
    Depositor
    Kan Wang
    Article
    Paz et al Plant Cell Rep. 2006 Mar;25(3):206-13. d
    Insert
    attR3-CmR-ccdB-attR4
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    lacUV5 promoter
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172181-plasmid-map-sequence-id-343829

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  5. pBJG1 T7-8xHis-OGT K842M

    Plasmid
    #154272
    Purpose
    Bacterial expression of full length human OGT with K842M mutation (catalytically inactive). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Lazarus et al Nature. 2011 Jan 27;469(7331):564-7. doi
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Lysine 842 changed to Methionine- bacterial codon…
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154272-plasmid-map-sequence-id-304833

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  7. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  8. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid
    #117699
    Purpose
    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    Use
    Tags
    HA tag, Myc-NES, and NLS
    Expression
    Mammalian
    Mutation
    codon optimized for murine expression
    Promoter
    None, 2A peptide from porcine teschovirus-1 polyp…
    Available since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    117699-plasmid-map-sequence-id-368109

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  10. SSN-GFP

    Plasmid
    #163663
    Purpose
    Expresses a TNF and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TNF in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1 and tumor necrosis factor (ST6GAL1, TNF Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains the cytosolic tail from ST,…
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163663-plasmid-map-sequence-id-322796

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  14. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Promoter
    Available since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

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  18. EGFP-uTEV1-MAVS-mTA4

    Plasmid
    #171002
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171002-plasmid-map-sequence-id-335829

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Showing: 401 - 420 of 5880 results