-
Plasmid#231622PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS3 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS6
Plasmid#231620PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS5
Plasmid#231619PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS4
Plasmid#231618PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS2 at R1 site and RBS1 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS3
Plasmid#231617PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS3 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVCS2
Plasmid#231616PurposeCumate and vanillate inducible toggle switch reported by mKate and/or sfGFP fluorescence signals; has RBS1 at R1 site and RBS2 at R2 site.DepositorInsertPcym-VanR-mKate2-Pvan-CymR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPvan/PcymAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS5
Plasmid#231614PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPbad-TetR-sfGFP-Ptet-AraC-mKate2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pS4
Plasmid#231613PurposeL-arabinose (Ara)- and anhydrotetracycline (aTc)-inducible inverter circuit reported by mKate and/or sfGFP fluorescence signalsDepositorInsertPtet-AraC-mKate2-Pbad-TetR-sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbad/PtetAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRN10
Plasmid#84454Purposeshuttle plasmid for sarA P1-mKate expressionDepositorInsertmKate2
UseShuttle vector e.coli-s.aureus, expression of sgf…TagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
TC374
Plasmid#164886PurposePrpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,DepositorInsertrpl28-rtTA(Q)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TC550
Plasmid#164891PurposePunc-4::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1DepositorInsertunc4-rtTA(Q)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSO5
Plasmid#106364PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr I.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP151
Plasmid#106359PurposeTo insert a single copy of a construct at a site on Chr II, corresponding to a mKate-labelled LifeAct expressed specifically in the adult epidermis.DepositorInsertcol-62p_lifeact_mkate2_3'nmy-2
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC562
Plasmid#164887PurposePunc-4::rtetR-QFAD(650-777+790-816)::P2A::mKate::T2A::tetR-pie1DepositorInsertQFAD mutated
UseTagsExpressionWormMutationPromoterAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTagRFP657-C1
Plasmid#31872PurposeFor fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.DepositorInsertTagRFP657
UseTagsExpressionMammalianMutationM44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…PromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-941
Plasmid#103757PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-941 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-941 target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Immune checkpoint (library 2) Backbone
Plasmid#192363PurposePlasmid vector for in vivo immune checkpoint screening library (for checkpoint library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
T cell activation (library 1) Backbone
Plasmid#192364PurposePlasmid vector for in vitro Jurkat activation screening (for immune library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only