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Plasmid#132372Purposecontains the sequence of mCerulean3 for C-term fusions in the pGGD000 backbone (GreenGate)DepositorInsertlinker-mCerulean3
UseGreengate entry plasmidTagsExpressionMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjVASA-tdTomato
Plasmid#160872PurposeMarmoset TargetingDepositorInserttdTomato
UseMarmoset targetingTagsExpressionMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK3_031
Plasmid#123745PurposeEncodes p38 Translocation Reporter (p38 KTR) fused to mCerulean3 as a Type 3 part to be used in the MTK systemDepositorInsertp38KTR-mCerulean3
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
tagSrc(WT)-mCer3 S208F/V224L
Plasmid#179138PurposemCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).DepositorInsertSrc, SsrA
UseTagsmCerulean3 (S208F/V224L)ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-5 in pcDNAI/Amp
Plasmid#55625PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-5. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-5 (GNG5 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-5 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-1 in pcDNAI/Amp
Plasmid#55623PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-1 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-2 in pcDNAI/Amp
Plasmid#55624PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-2. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-2 (Gng2 Aequorea victoria, Mouse)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-2 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-7 in pcDNAI/Amp
Plasmid#55626PurposeAn amino-terminal mCerulean fragment was fused to Ggamma-7. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-7 (GNG7 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-7 was amplified by PCR, which added a BamH…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-10 in pcDNAI/Amp
Plasmid#55627PurposeAn N-terminal mCerulean fragment was fused to Ggamma-10. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-10 (GNG10 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-10 was amplified by PCR, which added a Bam…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-11 in pcDNAI/Amp
Plasmid#55628PurposeAn N-terminal mCerulean fragment was fused to Ggamma-11. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-11 (GNG11 Bovine, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-11 was amplified by PCR, which added a Bam…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-gamma-12 in pcDNAI/Amp
Plasmid#55629PurposeAn N-terminal mCerulean fragment was fused to Ggamma-12. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.DepositorInsertCer(1-158)-gamma-12 (GNG12 Human, Aequorea victoria)
UseTagsCer(1-158) was fused to the amino terminus of Gga…ExpressionMammalianMutationGgamma-12 was amplified by PCR, which added a Bam…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-gamma-2 in pcDNAI/Amp
Plasmid#55777PurposeA C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.DepositorInsertCFP(159-238)-gamma-2 (GNG2 Human, Aequorea victoria)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)
Plasmid#156416Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseZebrafish transgenesisTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 858 (pTol2-Kdrl-V5-mScarlet-KRAS4A-WT--AC-Cerulean)
Plasmid#180468Purposekdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A with an alpha-crystallin mCerulean reporter flanked by Tol2 sitesDepositorInsertKRAS4A (KRAS Human)
UseZebrafish transgenesisTagsV5-mScarlet-IExpressionMutationPromoterkdrlAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2
Plasmid#125195PurposeSB-transposon plasmid for stable expression of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Puro_LPAR_AKAR-WT
Plasmid#125206PurposeSB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporterDepositorInsertLPAR_AKAR-WT
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-Lyn-AktAR2-EV
Plasmid#125199PurposeSB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporterDepositorInsertLyn-AktAR2-EV
UseTransposonTagsEV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…ExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-AktAR2-D
Plasmid#125196PurposeSB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporterDepositorInsertAktAR2-D
UseTransposonTags6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…ExpressionMammalianMutationFoxO1 Thr-24 changed to Val rendering this inacti…PromoterEF1a/RPBSAAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRD
Plasmid#172924PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRD
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_ionRE
Plasmid#172926PurposeIonic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.DepositorInsertIonicStrengthSensorRE
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only