Purpose
Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
Inserts
Left homology arm
Puromycin resistance gene
Venus fluorescence gene
Thymidine kinase
Right homology arm
Use
Cre/Lox; Homology recombination selection vector
Tags
Expression
Bacterial and Mammalian
Mutation
Promoter
EF1a promoter, EF1a promoter (T2A cleaving sequen…
Available since
Sept. 19, 2018
Availability
Academic Institutions and Nonprofits only