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RV-mCD4-HH5-4

Plasmid

#124984

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-4 TCRa P2A TCRb

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-3

Plasmid

#124983

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-3 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

TCR-HH5-3

Use

retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-ER-TurboID

Plasmid

#149415

Purpose

Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

ER-TurboID

Use

AAV

Tags

HA and V5

Expression

Mutation

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Expression

Mutation

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-puro HGF

Plasmid

#10901

Depositor

Bob Weinberg

Article

Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

Insert

HGF (HGF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD43-52

Plasmid

#98684

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

hepatds 43-52, residues 1888-1970

Promoter

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-hHNF4aORF-1

Plasmid

#206959

Purpose

The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the NheI/NotI sites of the pET21b(+), the bacterial expression vector.

Depositor

Reza Zolfaghari, Catharine Ross

Insert

HNF4a (Hepatocyte Nuclear Factor alpha4 Variant 2) (HNF4A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-mem-TurboID

Plasmid

#149413

Purpose

Hepatocyte-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

mem-TurboID

Use

AAV

Tags

HA and V5

Expression

Mutation

Promoter

TBG

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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KHBD00294

Plasmid

#34420

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG9310 (Hnf4 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCI-Neo-p53mut

Plasmid

#99239

Purpose

Mutant p53 encoded by AS-30D rat hepatoma cell line

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

Insert

p53 (Tp53 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations in translated protein at Gly (10…

Promoter

CMV immediate/early enhancer/promoter

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-GCaMP6f-3x-miR204-5p-3x-miR122-TS

Plasmid

#117384

Purpose

An AAV genome containing miRNA target sequences (TS) 204-5p and 122 to reduce expression of GCaMP6f in astrocytes and hepatocytes, respectively

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

GCaMP6f + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA ; 3 copies of miR122 : CAAACACCATTGTCACACTCCA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-Neo-HKIImut

Plasmid

#99240

Purpose

Type II Hexokinase expressed in AS-30D rat hepatoma cell line

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

Insert

Hexokinase II (Hk2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Four silent mutations; P (115)L, A (192) V, F (78…

Promoter

CMV I.E.

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-SV40NLSf-GFP-3xmiR122-WPRE-HGHpA

Plasmid

#183775

Purpose

AAV genome with a CAG driven eGFP reporter with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

NLS-GFP

Use

AAV

Tags

Expression

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Cre-3xmiR122-WPRE-HGHpA

Plasmid

#183776

Purpose

AAV genome with a CAG driven Cre recombinase with mR122 target sequence repeats to reduce transgene expression in hepatocytes.

Depositor

Ben Deverman

Article

Krolak et al Nat Cardiovasc Res. 2022 Apr;1(4):389-40

Insert

Cre

Use

AAV

Tags

Expression

Mutation

NA

Promoter

CAG

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pWAP-HGF

Plasmid

#83503

Purpose

Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

Depositor

Marta Gallego, Lothar Hennighausen

Article

Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

Insert

HGF (Hgf Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

also contains beta-globin sequences from pUC198

Promoter

mouse WAP (whey acidic protein)

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

Plasmid

#105127

Purpose

Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein

Depositor

Didier Stainier, Kimberley Evason

Article

Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d

Inserts

Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

cryaa (crystallin) and fabp10a

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-MET

Plasmid

#23889

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MET (MET Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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