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Plasmid#119867PurposeDonor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-Tyrosinase-bait-T2a-Cherry-pA
Plasmid#111155Purposeknock in bait for Axolotl Tyrosinase locusDepositorInsertTyrosinase-bait-T2a-Cherry-pA
UseTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEM-mouse TCR zeta/2451
Plasmid#11506DepositorInsertTCR zeta (Cd247 Mouse)
UseGeneral cloningTagsExpressionMutationn/aPromoterAvailable sinceMarch 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-PT2A-Tdtomato-GFP-iRFP670
Plasmid#111814PurposeTricistronic construct: Co-express three fluorescent proteins by P2A and then T2ADepositorInsertsTdtomato
GFP
iRFP670
UseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE169 mouse Wnt-1-myc-pGEM4
Plasmid#16855DepositorInsertWnt-1-myc (Wnt1 Mouse)
UseXenopus expressionTagsMycExpressionMutationPromoterAvailable sinceSept. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)
Plasmid#224754PurposeIn vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)DepositorInsertPotassium channel subfamily K member 2 (Kcnk2 Mouse)
UseIn vitro transcription for oocyte expressionTagsExpressionMutationC159A/C219A/C365A/C399APromoterT7Available sinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-298-5NS-GFP
Plasmid#205742PurposeExpresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O C-terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMMK ENTR 1 CMV mAG h-Geminin
Plasmid#206281PurposeENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMVDepositorInsertmAG-hGeminin (GMNN Human)
UseMultimate/gateway entr 1TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Cas9-nls-CDS1
Plasmid#160231PurposeCas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1DepositorInsertCas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
UseLevel 0 of moclo golden gate position cds1TagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP1-loxP-bar-lox2272-71
Plasmid#168785PurposeVector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGem-LP2-loxP-bar-lox2272-71
Plasmid#168786PurposeVector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.DepositorInsertsbar
IS1 Homology arm 1
IS1 Homology arm 2
loxP
lox2272-71
UseCre/Lox and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-Isce-Tol2-Tn5-neo
Plasmid#176619Purposefor generating PCR templates with Tol2 repeats and Isce sites for BAC recombinationDepositorInsertI-SceI sites, Tol2 repeats and Tn5-neo cassette
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-bPAC(R278A)-Myc-eYFP
Plasmid#165488PurposeXenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activityDepositorInsertbPAC(R278A)-Myc-eYFP
UseCdna expression, xenopus oocyteTagsEYFP and MycExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM-myr-Citrine-SV40pA-FKF
Plasmid#119865PurposeDonor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertmyr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTags2x myristoylationExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast
Plasmid#197066PurposeDonor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBPDepositorInsertEncapsulin
UseCRISPRTagsFKBP and HaloTagExpressionMammalianMutationCodon-optimised for human cell expressionPromoterAvailable sinceSept. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
Plasmid#71707PurposeA human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmidDepositorInserthumanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
UseCRISPRTags3xFLAG and hGEM(1/110)ExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5
Plasmid#111818PurposeQuadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2ADepositorUseCloning intermediateTagsExpressionMutationPromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro GEM-LTB4
Plasmid#202641PurposeExpresses the LTB4 sensor GEM-LTB4 in mammalian cellsDepositorInsertGEM-LTB4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro GEM-LTB4mut
Plasmid#202642PurposeExpresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cellsDepositorInsertGEM-LTB4mut
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)
Plasmid#165489PurposeXenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus-bPAC(F198Y)
UseCdna expression, xenopus oocyteTagsVenusExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only