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Plasmid#148565PurposeBacterial Expression of Hs4EHP_52-234opt-R103LE149LDepositorInsertHs4EHP_52-234opt-R103LE149L (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AE
Plasmid#148567PurposeBacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202EDepositorInsertHs4EHP_52-234opt-Q159SM161DR202E (EIF4E2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGL4-4X TE Down (GQs) Renilla
Plasmid#70039PurposePlasmid for testing 5’UTR TE Down (G-quadruplex) folding elementsDepositorInsert4x TE Down (G-quadruplexes) in 5'UTR
UseLuciferaseTagsrenilla luciferaseExpressionMammalianMutation4 repeats of TE down motif (G-quadruplexes) in 5&…PromoterSV40Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmeEF1A_R
Plasmid#147347PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeEF1A_R
Plasmid#147349PurposeInsect Expression of DmeIF1ADepositorInsertDmeIF1A (eIF-1A Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-Hs4E-T_AA
Plasmid#148233PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
synIRES_RC25
Plasmid#189970PurposePart 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAsDepositorInsertsynIRES_RC25
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I F113A
Plasmid#15680DepositorInsertPHAS-I F113A (Eif4ebp1 Rat)
UseTags6xHisExpressionBacterialMutationF113A mutation.PromoterAvailable sinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
UseTags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3…PromoterAvailable sinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR(T/A)
Plasmid#64928PurposeNegative-control mutant for TORCAR biosensor.DepositorInsertTORCAR(T/A) (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutation4EBP1 domain contains T37A and T46A mutations.PromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR
Plasmid#64927PurposeFRET-based biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean and YPetExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1 M161A/D165K
Plasmid#65809Purposeexpression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4GDepositorInsertPABPC1 (PABPC3 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationM161A/D165KPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUPERpuro-BglII-Hs4E-T_AC
Plasmid#148445PurposeMammalian Expression of Hs4E-T-shRNADepositorInsertHs4E-T-shRNA (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PM-TORCAR
Plasmid#64930PurposePlasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertLyn-TORCAR (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-Hs4E-T_AF
Plasmid#148687PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TORCAR-NLS
Plasmid#64931PurposeNuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.DepositorInsertTORCAR-NLS (EIF4EBP1 Human, Synthetic)
UseTagsCerulean, Nuclear localization signal (NLS), and …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-L61AV67A_Z
Plasmid#148147PurposeMammalian Expression of Hs4EBP3-L61AV67ADepositorInsertHs4EBP3-L61AV67A (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-Y40AL45A_Z
Plasmid#148148PurposeMammalian Expression of Hs4EBP3-Y40AL45ADepositorInsertHs4EBP3-Y40AL45A (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP3-YLLVtoAAAA_Z
Plasmid#148149PurposeMammalian Expression of Hs4EBP3-YLLVtoAAAADepositorInsertHs4EBP3-YLLVtoAAAA (EIF4EBP3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-Hs4EBP1-L75AV81A_Z
Plasmid#148144PurposeMammalian Expression of Hs4EBP1-L75AV81ADepositorInsertHs4EBP1-L75AV81A (EIF4EBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only