-
Plasmid#56614PurposeLocalization: Centrosomes, Excitation: 515, Emission: 528DepositorInsertGamma-Tubulin (TUBG1 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFX-Lifeact-Venus
Plasmid#174478PurposeExpress Venus with actin-targeting sequence in mammalian cellsDepositorInsertVenus with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-Venus-cdc25C1
Plasmid#40968DepositorInsertcdc25C5 (CDC25C Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mVenus-Beta-Catenin-20
Plasmid#56604PurposeLocalization: Tight Junctions, Excitation: 515, Emission: 528DepositorInsertBeta-Catenin (Ctnnb1 Mouse)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFX-GalT-Venus
Plasmid#174472PurposeExpress Venus with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertVenus with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-KRasCT
Plasmid#175834PurposeExpress Venus with plasma membrane-targeting sequence in mammalian cellsDepositorInsertVenus with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Tom20-Venus
Plasmid#174187PurposeExpress Venus with mitochondria-targeting sequence in mammalian cellsDepositorInsertVenus with mitchondria-targeting sequence
UseTagsthe mitochondria-targeting sequence of TOM20ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-G protein Gamma 5
Plasmid#42200DepositorInsertGamma 5 (Gng5 Rat)
UseTagsVenus(1-155)ExpressionMammalianMutationcodon-optimizedPromoterCMVAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-cpVenus-Metallothionein-3
Plasmid#58176PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagscpVenus-Metallothionein and mCeruleanExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN15-mVenus
Plasmid#168486PurposeMammalian expression of the N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe N-terminal 15 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cerulean-Venus-FRET-10
Plasmid#58177PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsCerulean and VenusExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
CaM-RhoA(DP)-Venus
Plasmid#31994DepositorInsertCaM-RhoA(DP) (RHOA Human)
UseTagsYFPExpressionBacterial and MammalianMutationQ63L mutation in RhoA, E7S and D65Y in CaMPromoterAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVenus-PICK1-shRNA
Plasmid#72575PurposeExpresses shRNA against PICK1 as well as the marker Venus fluorescent proteinDepositorUseTagsExpressionMammalianMutationPromoterH1 promoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Scarlet I-FRET 10-Venus
Plasmid#139366PurposeFRET StandardDepositorInsertScarlet 10AA Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.A184V
Plasmid#106196PurposeMedium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.A184V
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: A184VPromoterGFAPAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SF-Venus-iGluSnFR.S72A
Plasmid#106197PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-Venus-iGluSnFR.S72A
UseAAVTagsExpressionMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterGFAPAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKK-mVenus-TEV
Plasmid#105774PurposeExpression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).DepositorTypeEmpty backboneUseFlp-in competentTagsmVenus-TEVExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
mVenus-PKCB1 K371R
Plasmid#195230PurposeRetroviral vector containing GFP tagged PKCB1. Kinase inactive mutant.DepositorInsertPKCB1 (PRKCB Human)
UseRetroviralTagsExpressionMutationK371RPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-Venus-His8
Plasmid#223889Purpose4G-cloning donor for C-terminal 3C-Venus-His8 tagDepositorInsert3C-Venus-His8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only