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Plasmid#188496PurposeExpresses FLAG tagged PlmCas12eDepositorInsertCas12e
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
DpbdCas12e KRAB
Plasmid#188505PurposeExpresses FLAG tagged DpbdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsExpressionMammalianMutationD672A/E769A/D935APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cas12j3
Plasmid#188497PurposeExpresses FLAG tagged Cas12j3DepositorInsertCas12j3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD187 Tri-AND Gate, 2 repeats
Plasmid#138940PurposeEYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoterDepositorInsertLogic 37 AND 43 AND 158 2 repeats
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 43 AND 158 2 repeatsAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD186 Tri-AND Gate, 1 repeat
Plasmid#138939PurposeEYFP reporter vector for the Logic 37 AND 43 AND 158 1 repeat promoterDepositorInsertLogic 37 AND 43 AND 158 1 repeat
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 43 AND 158 1 repeatAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD129 AND Gate, 4 repeats
Plasmid#138938PurposeEYFP reporter vector for the Logic 37 AND 158 NOT 43 4 repeats promoterDepositorInsertLogic 37 AND 158 NOT 43 4 repeats
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 158 NOT 43 4 repeatsAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD128 AND Gate, 3 repeats
Plasmid#138937PurposeEYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoterDepositorInsertLogic 37 AND 158 NOT 43 3 repeats
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 158 NOT 43 3 repeatsAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD127 AND Gate, 2 repeats
Plasmid#138936PurposeEYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoterDepositorInsertLogic 37 AND 158 NOT 43 2 repeats
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 158 NOT 43 2 repeatsAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD126 AND Gate, 1 repeat
Plasmid#138935PurposeEYFP reporter vector for the Logic 37 AND 158 NOT 43 1 repeat promoterDepositorInsertLogic 37 AND 158 NOT 43 1 repeat
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterLogic 37 AND 158 NOT 43 1 repeatAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD270 ZF43x6-Compact(overlapping ZF37)_EYFP
Plasmid#138934PurposeEYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoterDepositorInsertZF43x6-Compact(overlapping ZF37)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF43x6-Compact(overlapping ZF37)Available sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
YSBE420:pNEO_CAC1_Vn_HOG1 terminator
Plasmid#122701PurposePlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus proteinDepositorInsertN-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
YSBE418:pNAT_ACA1_Vc_HOG1 terminator
Plasmid#122700PurposePlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus proteinDepositorInsertC-terminal fragment of Venus protein
UseTagging proteins in cryptococcus neoformansTagsExpressionMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgBFP
Plasmid#192478PurposeAll-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3DepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_mCherry sgAAVS1
Plasmid#192480PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1 or V2 cells as controlDepositorInsertSpCas9
UseTagsT2A mCherryExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-hU6-evopreQ1
Plasmid#210189PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV2-mU6-evopreQ1
Plasmid#210190PurposepegRNA/ngRNA library cloningDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW212-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-mScarlet-I-P2A-BlastR
Plasmid#170810PurposeLentiviral vector to co-express an spsgRNA with NLS-mScarlet-IDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-APOBEC1-YTHmut
Plasmid#178950PurposeLentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cellsDepositorInsertAPOBEC1-YTHmut-T2A-GFP
UseLentiviral and Synthetic Biology; InducibleTagsHAExpressionMammalianMutationPromotertight TREAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
HiFi dCas9 VPR
Plasmid#188510PurposeExpresses FLAG tagged HiFi dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/R691A/H840APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only