Skip to main content
Addgene
Showing: 401 - 420 of 706 results

  1. pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

    Plasmid
    #201539
    Purpose
    Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)
    Depositor
    Joshua Leonard
    Article
    Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:
    Inserts
    PuromycinR-P2A-miRFP720
    ZF6x12-C-DsRed-Express2(x6)
    BlasticidinR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…
    Available since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    201539-plasmid-map-sequence-id-401324

    Enlarge

  9. ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

    Plasmid
    #179586
    Purpose
    ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)
    Depositor
    Ali Hemmati Brivanlou
    Article
    De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:
    Insert
    NLS-NG-T2A-hSHH
    Use
    Piggybac
    Tags
    NeonGreen
    Expression
    Mutation
    Promoter
    TRE
    Available since
    Feb. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    179586-plasmid-map-sequence-id-354597

    Enlarge

  14. 1073A(HomeR1)=pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159676
    Purpose
    Plasmid provides the HomeR#1 gene drive element harboring a rescue, gRNA#1, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #1 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#1(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159676-plasmid-map-sequence-id-314890

    Enlarge

  15. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

    Plasmid
    #159677
    Purpose
    Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.
    Depositor
    Omar Akbari
    Article
    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1
    Insert
    [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    159677-plasmid-map-sequence-id-314891

    Enlarge

  16. pJAT7

    Plasmid
    #204292
    Purpose
    For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204292-plasmid-map-sequence-id-405844

    Enlarge

Showing: 401 - 420 of 706 results