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Plasmid#199484PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertGal4vp16/4xnrUAS-mKate2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKAM-E-Flag-BFP
Plasmid#101867PurposeLentiviral Evi-1-Flag with dual BFP and puro markersDepositorInsertEvi-1 (Mecom Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
TagBFP-mEmerald-FRET
Plasmid#58159PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsTagBFP and mEmeraldExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mTagBFP2-mEmerald-FRET
Plasmid#58163PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagsmEmerald and mTagBFP2ExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TagBFP2-C1-FKB-PI-PLCN187
Plasmid#139307PurposeN-terminal half of split PI-PLCDepositorInsertTagBFP2:FKBP1A(3-108):[GGSA]4GG:L. monocytogenes PI-PLC(1-187)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLI_BFP_C-tag TNF
Plasmid#171187Purposeinducible expression of C-tag TNFDepositorInsertC-tag TNF
UseLentiviralTagsC-tag (internal)ExpressionMammalianMutationADAM cleavage site of TNF (AA73-76) was exchanged…Promotertight TRE promoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-QCP-3xBFP
Plasmid#158206PurposeQb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.DepositorInsertQCP under the Elfa promoter and three BFP tags
UseSynthetic BiologyTags3xBFPExpressionMammalianMutationPromoterElfaAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-blast-BFP
Plasmid#190258PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertBFP
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1-TelR15-mTagBFP2
Plasmid#49643PurposeTALEBlue-Telomere for In Vitro TranslationDepositorInsertTelR15
UseIn vitro translationTagsmTagBFP2ExpressionMutationPromoterT7Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ManII-SBP-tagBFP
Plasmid#65257Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertIi-Streptavidin and truncated Mannosidase II
UseTagsStreptavidin Binding Protein (SBP) and tagBFPExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-FKBP-CYB5Atail
Plasmid#108141PurposeFor recruitment of ERDepositorUseTagsExpressionMammalianMutationCYB5A(100-134)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-AtAFB2-mTagBFP2
Plasmid#179889PurposeExpresses AtAFB2 (auxin-inducible degron system) fused to mTagBFP2 (lentiviral plasmid)DepositorInsertAtAFB2 (AFB2 Mustard Weed, Synthetic)
UseLentiviralTagsmTagBFP2ExpressionBacterial and MammalianMutationPromoterEF1Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK-TEV-mTagBFP
Plasmid#105789PurposeExpression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsTEV-mTagBFPExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hNCL
Plasmid#103805PurposeBLInCR 'Localizer' construct that marks the nucleoli and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhNCL: A395G (K132R), A421G (K141E), A424G (K142E)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR Fyn-TagBFP
Plasmid#171035PurposeTagBFP-tagged Fyn kinaseDepositorInsertFyn
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS2-his-24::tagBFP
Plasmid#124320PurposeExpressing Nuclear Localized tagBFPDepositorInserttagBFP (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRVdG-N-P-M-nucBFP-EGFP-L
Plasmid#52485PurposeExpresses nuclear mTagBFP and EGFPDepositorInsertsnuclear mTagBFP
EGFP
UseRabies virusTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA-TagBFP
Plasmid#120910PurposeExpresses BFP tagged Ptch1-BDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsHA and TagBFPExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(P6f)3(P14f)3-gs
Plasmid#228081PurposeMammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, P6f, P14f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only